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{{STRUCTURE_4ii9|  PDB=4ii9  |  SCENE=  }}
==Crystal structure of Weissella viridescens FemXVv non-ribosomal amino acid transferase in complex with a peptidyl-RNA conjugate==
===Crystal structure of Weissella viridescens FemXVv non-ribosomal amino acid transferase in complex with a peptidyl-RNA conjugate===
<StructureSection load='4ii9' size='340' side='right' caption='[[4ii9]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
{{ABSTRACT_PUBMED_23744707}}
== Structural highlights ==
<table><tr><td colspan='2'>[[4ii9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Weissella_viridescens Weissella viridescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4II9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4II9 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MUB:N-ACETYLMURAMIC+ACID'>MUB</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A9Z:2-DEOXY-2-(4-ETHYL-1H-1,2,3-TRIAZOL-1-YL)ADENOSINE+5-(DIHYDROGEN+PHOSPHATE)'>A9Z</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=FGA:GAMMA-D-GLUTAMIC+ACID'>FGA</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ne9|1ne9]], [[1p4n|1p4n]], [[1xe4|1xe4]], [[1xf8|1xf8]], [[1xix|1xix]], [[3gkr|3gkr]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">femX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1629 Weissella viridescens])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylmuramoylpentapeptide-lysine_N(6)-alanyltransferase UDP-N-acetylmuramoylpentapeptide-lysine N(6)-alanyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.10 2.3.2.10] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ii9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ii9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ii9 RCSB], [http://www.ebi.ac.uk/pdbsum/4ii9 PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
To gain insight into the catalytic mechanism of non-ribosomal amino acid transferases, peptidyl-RNA conjugates were synthesized for co-crystallization with FemXWv of Weissella viridescens, which transfers L-Ala from Ala-tRNAAla to the peptidoglycan precursor UDP-MurNAc-pentapeptide. The structure of the resulting complex and mutational studies revealed the mechanism by which FemXWv binds its substrates for substrate-assisted catalysis and stabilization of the tetrahedral intermediate.


==About this Structure==
The Structure of FemX in Complex with a Peptidyl-RNA Conjugate: Mechanism of Aminoacyl Transfer from Ala-tRNA to Peptidoglycan Precursors.,Fonvielle M, Li de La Sierra-Gallay I, El-Sagheer AH, Lecerf M, Patin D, Mellal D, Mayer C, Blanot D, Gale N, Brown T, van Tilbeurgh H, Etheve-Quelquejeu M, Arthur M Angew Chem Int Ed Engl. 2013 Jun 6. doi: 10.1002/anie.201301411. PMID:23744707<ref>PMID:23744707</ref>
[[4ii9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Weissella_viridescens Weissella viridescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4II9 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:023744707</ref><references group="xtra"/><references/>
</div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Weissella viridescens]]
[[Category: Weissella viridescens]]
[[Category: Arthur, M.]]
[[Category: Arthur, M]]
[[Category: Etheve-Quelquejeu, M.]]
[[Category: Etheve-Quelquejeu, M]]
[[Category: Fonvielle, M.]]
[[Category: Fonvielle, M]]
[[Category: Sierra-Gallay, I Li de la.]]
[[Category: Sierra-Gallay, I Li de la]]
[[Category: Tilbeurgh, H van.]]
[[Category: Tilbeurgh, H van]]
[[Category: Femx]]
[[Category: Femx]]
[[Category: Peptidoglycan]]
[[Category: Peptidoglycan]]
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