2ih1: Difference between revisions
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[[Image:2ih1.gif|left|200px]] | [[Image:2ih1.gif|left|200px]] | ||
'''Ion selectivity in a semi-synthetic K+ channel locked in the conductive conformation''' | {{Structure | ||
|PDB= 2ih1 |SIZE=350|CAPTION= <scene name='initialview01'>2ih1</scene>, resolution 2.40Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene> and <scene name='pdbligand=1EM:(1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL MYRISTATE'>1EM</scene> | |||
|ACTIVITY= | |||
|GENE= kcsA, skc1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1916 Streptomyces lividans]) | |||
}} | |||
'''Ion selectivity in a semi-synthetic K+ channel locked in the conductive conformation''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2IH1 is a [ | 2IH1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IH1 OCA]. | ||
==Reference== | ==Reference== | ||
Ion selectivity in a semisynthetic K+ channel locked in the conductive conformation., Valiyaveetil FI, Leonetti M, Muir TW, Mackinnon R, Science. 2006 Nov 10;314(5801):1004-7. PMID:[http:// | Ion selectivity in a semisynthetic K+ channel locked in the conductive conformation., Valiyaveetil FI, Leonetti M, Muir TW, Mackinnon R, Science. 2006 Nov 10;314(5801):1004-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17095703 17095703] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: ion channel d-amino acid semi-synthetic]] | [[Category: ion channel d-amino acid semi-synthetic]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:29:29 2008'' | ||
Revision as of 15:29, 20 March 2008
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| 2ih1, resolution 2.40Å | |||||||||||||
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| Ligands: | K and 1EM | ||||||||||||
| Gene: | kcsA, skc1 (Streptomyces lividans) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Ion selectivity in a semi-synthetic K+ channel locked in the conductive conformation
Overview
Potassium channels are K+-selective protein pores in cell membrane. The selectivity filter is the functional unit that allows K+ channels to distinguish potassium (K+) and sodium (Na+) ions. The filter's structure depends on whether K+ or Na+ ions are bound inside it. We synthesized a K+ channel containing the d-enantiomer of alanine in place of a conserved glycine and found by x-ray crystallography that its filter maintains the K+ (conductive) structure in the presence of Na+ and very low concentrations of K+. This channel conducts Na+ in the absence of K+ but not in the presence of K+. These findings demonstrate that the ability of the channel to adapt its structure differently to K+ and Na+ is a fundamental aspect of ion selectivity, as is the ability of multiple K+ ions to compete effectively with Na+ for the conductive filter.
About this Structure
2IH1 is a Single protein structure of sequence from Mus musculus and Streptomyces lividans. Full crystallographic information is available from OCA.
Reference
Ion selectivity in a semisynthetic K+ channel locked in the conductive conformation., Valiyaveetil FI, Leonetti M, Muir TW, Mackinnon R, Science. 2006 Nov 10;314(5801):1004-7. PMID:17095703
Page seeded by OCA on Thu Mar 20 17:29:29 2008
