4j8s: Difference between revisions
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==Crystal structure of human CNOT1 MIF4G domain in complex with a TTP peptide== | |||
<StructureSection load='4j8s' size='340' side='right' caption='[[4j8s]], [[Resolution|resolution]] 1.55Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4j8s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J8S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J8S FirstGlance]. <br> | |||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AD-005, CDC39, CNOT1, KIAA1007, NOT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j8s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j8s RCSB], [http://www.ebi.ac.uk/pdbsum/4j8s PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Tristetraprolin (TTP) is an RNA-binding protein that controls the inflammatory response by limiting the expression of several proinflammatory cytokines. TTP post-transcriptionally represses gene expression by interacting with AU-rich elements (AREs) in 3' untranslated regions of target mRNAs and subsequently engenders their deadenylation and decay. TTP accomplishes these tasks, at least in part, by recruiting the multisubunit CCR4-NOT deadenylase complex to the mRNA. Here we identify an evolutionarily conserved C-terminal motif in human TTP that directly binds a central domain of CNOT1, a core subunit of the CCR4-NOT complex. A high-resolution crystal structure of the TTP-CNOT1 complex was determined, providing the first structural insight, to our knowledge, into an ARE-binding protein bound to the CCR4-NOT complex. Mutations at the CNOT1-TTP interface impair TTP-mediated deadenylation, demonstrating the significance of this interaction in TTP-mediated gene silencing. | |||
Structural basis for the recruitment of the human CCR4-NOT deadenylase complex by tristetraprolin.,Fabian MR, Frank F, Rouya C, Siddiqui N, Lai WS, Karetnikov A, Blackshear PJ, Nagar B, Sonenberg N Nat Struct Mol Biol. 2013 Jun;20(6):735-9. doi: 10.1038/nsmb.2572. Epub 2013 May , 5. PMID:23644599<ref>PMID:23644599</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
== | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Blackshear, P J | [[Category: Blackshear, P J]] | ||
[[Category: Fabian, M R | [[Category: Fabian, M R]] | ||
[[Category: Frank, F | [[Category: Frank, F]] | ||
[[Category: Karetnikov, A | [[Category: Karetnikov, A]] | ||
[[Category: Lai, W S | [[Category: Lai, W S]] | ||
[[Category: Nagar, B | [[Category: Nagar, B]] | ||
[[Category: Rouya, C | [[Category: Rouya, C]] | ||
[[Category: Siddiqui, N | [[Category: Siddiqui, N]] | ||
[[Category: Sonenberg, N | [[Category: Sonenberg, N]] | ||
[[Category: Cytosol]] | [[Category: Cytosol]] | ||
[[Category: Mif4g]] | [[Category: Mif4g]] | ||
Revision as of 12:37, 21 December 2014
Crystal structure of human CNOT1 MIF4G domain in complex with a TTP peptide
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