RNase A: Difference between revisions
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== '''Introduction''' == | == '''Introduction''' == | ||
[[Image:RNaseAIII.png|300px|left|thumb|Figure I: Bovine Ribonuclease A. Colored residues are representative of amino acids important to both the acid base catalysis (Red: His12 and 119) and stabilization of the transition state (Blue: Lys41). Figure generated via ''Pymol'' ]] | [[Image:RNaseAIII.png|300px|left|thumb|Figure I: Bovine Ribonuclease A. Colored residues are representative of amino acids important to both the acid base catalysis (Red: His12 and 119) and stabilization of the transition state (Blue: Lys41). Figure generated via ''Pymol'' ]] | ||
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Ribonucleases or RNA depolymerases are enzymes that catalyze RNA degradation.<ref name="Raines"> PMID:11848924</ref> Ribonucleases are highly active in ruminants, such as cows, to digest large amounts of RNA produced by microorganisms in the stomach. Ruminants also have high amounts of ribonucleases to process nutrients from cellulose. One such ribonuclease, bovine pancreatic ribonuclease A or RNase A, was one of the most studied enzymes of the 20th century and was used as a model enzyme for many important findings in molecular science. <ref name="Raines" /> RNase A has been used as a foundational enzyme for the study of protein structure and function, molecular evolution, enzymatic catalysis, protein folding, protein semisynthesis, protein NMR spectroscropy, and protein oligomerization. RNase A is amenable to all of these areas due to its stability, small size, and because the three-dimensional structure is fully determined by its amino acid sequence.<ref name="Raines" /> | Ribonucleases or RNA depolymerases are enzymes that catalyze RNA degradation.<ref name="Raines"> PMID:11848924</ref> Ribonucleases are highly active in ruminants, such as cows, to digest large amounts of RNA produced by microorganisms in the stomach. Ruminants also have high amounts of ribonucleases to process nutrients from cellulose. One such ribonuclease, bovine pancreatic ribonuclease A or RNase A, was one of the most studied enzymes of the 20th century and was used as a model enzyme for many important findings in molecular science. <ref name="Raines" /> RNase A has been used as a foundational enzyme for the study of protein structure and function, molecular evolution, enzymatic catalysis, protein folding, protein semisynthesis, protein NMR spectroscropy, and protein oligomerization. RNase A is amenable to all of these areas due to its stability, small size, and because the three-dimensional structure is fully determined by its amino acid sequence.<ref name="Raines" /> | ||
