4j7o: Difference between revisions
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==Structure of the N-terminal Repeat Domain of Rickettsia Sca2== | |||
<StructureSection load='4j7o' size='340' side='right' caption='[[4j7o]], [[Resolution|resolution]] 2.17Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4j7o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rickettsia_conorii Rickettsia conorii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J7O FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sca2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=781 Rickettsia conorii])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j7o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j7o RCSB], [http://www.ebi.ac.uk/pdbsum/4j7o PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Sca2 (surface cell antigen 2) is the only bacterial protein known to promote both actin filament nucleation and profilin-dependent elongation, mimicking eukaryotic formins to assemble actin comet tails for Rickettsia motility. We show that Sca2's functional mimicry of formins is achieved through a unique mechanism. Unlike formins, Sca2 is monomeric, but has N- and C-terminal repeat domains (NRD and CRD) that interact with each other for processive barbed-end elongation. The crystal structure of NRD reveals a previously undescribed fold, consisting of helix-loop-helix repeats arranged into an overall crescent shape. CRD is predicted to share this fold and might form together with NRD, a doughnut-shaped formin-like structure. In between NRD and CRD, proline-rich sequences mediate the incorporation of profilin-actin for elongation, and WASP-homology 2 (WH2) domains recruit actin monomers for nucleation. Sca2's alpha-helical fold is unusual among Gram-negative autotransporters, which overwhelmingly fold as beta-solenoids. Rickettsia has therefore "rediscovered" formin-like actin nucleation and elongation. | |||
Rickettsia Sca2 has evolved formin-like activity through a different molecular mechanism.,Madasu Y, Suarez C, Kast DJ, Kovar DR, Dominguez R Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):E2677-86. doi:, 10.1073/pnas.1307235110. Epub 2013 Jul 1. PMID:23818602<ref>PMID:23818602</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Rickettsia conorii]] | [[Category: Rickettsia conorii]] | ||
[[Category: Dominguez, R | [[Category: Dominguez, R]] | ||
[[Category: Madasu, Y | [[Category: Madasu, Y]] | ||
[[Category: Actin]] | [[Category: Actin]] | ||
[[Category: Actin nucleation]] | [[Category: Actin nucleation]] | ||
[[Category: Cell invasion]] | [[Category: Cell invasion]] | ||
[[Category: Helical repeat]] | [[Category: Helical repeat]] | ||
Revision as of 12:02, 21 December 2014
Structure of the N-terminal Repeat Domain of Rickettsia Sca2
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