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{{STRUCTURE_4bjy|  PDB=4bjy  |  SCENE=  }}
==Crystal structure of 3-hydroxybenzoate 6-hydroxylase uncovers lipid- assisted flavoprotein strategy for regioselective aromatic hydroxylation: Platinum derivative==
===Crystal structure of 3-hydroxybenzoate 6-hydroxylase uncovers lipid- assisted flavoprotein strategy for regioselective aromatic hydroxylation: Platinum derivative===
<StructureSection load='4bjy' size='340' side='right' caption='[[4bjy]], [[Resolution|resolution]] 1.52&Aring;' scene=''>
{{ABSTRACT_PUBMED_23864660}}
== Structural highlights ==
<table><tr><td colspan='2'>[[4bjy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodococcus_jostii_rha1 Rhodococcus jostii rha1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BJY FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=P3A:PHOSPHATIDYLGLYCEROL-PHOSPHOGLYCEROL'>P3A</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bjz|4bjz]], [[4bk1|4bk1]], [[4bk2|4bk2]], [[4bk3|4bk3]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bjy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bjy RCSB], [http://www.ebi.ac.uk/pdbsum/4bjy PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
3-Hydroxybenzoate hydroxylase from Rhodococcus jostii RHA1 (3HB6H) is a dimeric flavoprotein that catalyses the NADH- and oxygen-dependent para-hydroxylation of 3 hydroxybenzoate to 2,5-dihydroxybenzoate. In this study, we report the crystal structure of 3HB6H, as expressed in Escherichia coli. The overall fold of 3HB6H is similar to that of p- hydroxybenzoate hydroxylase (PHBH) and other flavoprotein aromatic hydroxylases. Unexpectedly, a lipid ligand is bound to each 3HB6H monomer. Mass spectral analysis identifies the ligand as a mixture of phosphatidylglycerol and phosphatidylethanolamine. The fatty acid chains occupy hydrophobic channels that deeply penetrate into the interior of the substrate-binding domain of each subunit, whereas the hydrophilic part is exposed on the protein surface, connecting the dimerization domains via few interactions. Most remarkably, the terminal part of a phospholipid acyl chain is directly involved in the substrate-binding site. Co-crystallized chloride ion and the crystal structure of the His213Ser variant with bound 3-hydroxybenzoate provide hints about oxygen activation and substrate hydroxylation. Essential roles are played by His213 in catalysis and Tyr105 in substrate binding. This phospholipid-assisted strategy to control regioselective aromatic hydroxylation is of relevance for optimization of flavin-dependent biocatalysts.


==About this Structure==
Crystal structure of 3-hydroxybenzoate 6-hydroxylase uncovers lipid-assisted flavoprotein strategy for regioselective aromatic hydroxylation.,Montersino S, Orru R, Barendregt A, Westphal AH, van Duijn E, Mattevi A, van Berkel WJ J Biol Chem. 2013 Jul 17. PMID:23864660<ref>PMID:23864660</ref>
[[4bjy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodococcus_jostii_rha1 Rhodococcus jostii rha1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BJY OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:023864660</ref><references group="xtra"/><references/>
</div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Rhodococcus jostii rha1]]
[[Category: Rhodococcus jostii rha1]]
[[Category: Barendregt, A.]]
[[Category: Barendregt, A]]
[[Category: Berkel, W J.H van.]]
[[Category: Berkel, W J.H van]]
[[Category: Duijn, E van.]]
[[Category: Duijn, E van]]
[[Category: Mattevi, A.]]
[[Category: Mattevi, A]]
[[Category: Montersino, S.]]
[[Category: Montersino, S]]
[[Category: Orru, R.]]
[[Category: Orru, R]]
[[Category: Westphal, A H.]]
[[Category: Westphal, A H]]
[[Category: Gentisate]]
[[Category: Gentisate]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Phospholipid]]
[[Category: Phospholipid]]
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