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{{STRUCTURE_4hhw|  PDB=4hhw | SCENE= }}
==Crystal structure of the Pseudomonas aeruginosa azurin, H124NO YOH122==
===Crystal structure of the Pseudomonas aeruginosa azurin, H124NO YOH122===
<StructureSection load='4hhw' size='340' side='right' caption='[[4hhw]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
{{ABSTRACT_PUBMED_23859602}}
== Structural highlights ==
<table><tr><td colspan='2'>[[4hhw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HHW FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NIY:META-NITRO-TYROSINE'>NIY</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hhg|4hhg]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">azu, PA4922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 Pseudomonas aeruginosa PAO1])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hhw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hhw RCSB], [http://www.ebi.ac.uk/pdbsum/4hhw PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We have designed ruthenium-modified Pseudomonas aeruginosa azurins that incorporate 3-nitrotyrosine (NO2YOH) between Ru(2,2'-bipyridine)2(imidazole)(histidine) and Cu redox centers in electron transfer (ET) pathways. We investigated the structures and reactivities of three different systems: RuH107NO2YOH109, RuH124NO2YOH122, and RuH126NO2YOH122. RuH107NO2YOH109, unlabeled H124NO2YOH122, and unlabeled H126NO2YOH122 were structurally characterized. The pKa's of NO2YOH at positions 122 and 109 are 7.2 and 6.0, respectively. Reduction potentials of 3-nitrotyrosinate (NO2YO-)-modified azurins were estimated from cyclic and differential pulse voltammetry data: oxidation of NO2YO-122 occurs near 1.1 versus NHE; oxidation of NO2YO-109 is near 1.2 V. Our analysis of transient optical spectroscopic experiments indicates that hopping via NO2YO- enhances CuI oxidation rates over single-step ET by factors of 32 (RuH107NO2YO-109), 46 (RuH126NO2YO-122), and 13 (RuH124NO2YO-122).


==Function==
Electron Flow through Nitrotyrosinate in Pseudomonas aeruginosa Azurin.,Warren JJ, Herrera N, Hill MG, Winkler JR, Gray HB J Am Chem Soc. 2013 Jul 16. PMID:23859602<ref>PMID:23859602</ref>
[[http://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE]] Transfers electrons from cytochrome c551 to cytochrome oxidase.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[4hhw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HHW OCA].
</div>


==Reference==
==See Also==
<ref group="xtra">PMID:023859602</ref><references group="xtra"/><references/>
*[[Azurin|Azurin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Pseudomonas aeruginosa pao1]]
[[Category: Pseudomonas aeruginosa pao1]]
[[Category: Gray, H B.]]
[[Category: Gray, H B]]
[[Category: Herrera, N.]]
[[Category: Herrera, N]]
[[Category: Warren, J J.]]
[[Category: Warren, J J]]
[[Category: Electron transfer]]
[[Category: Electron transfer]]
[[Category: Electron transport]]
[[Category: Electron transport]]
[[Category: Greek key]]
[[Category: Greek key]]
[[Category: Nitrosylated]]
[[Category: Nitrosylated]]
Retrieved from "https://proteopedia.org/4hhw"