4lf0: Difference between revisions

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'''Unreleased structure'''
{{STRUCTURE_4lf0|  PDB=4lf0  |  SCENE=  }}
===The E142D mutant of the amidase from Geobacillus pallidus===


The entry 4lf0 is ON HOLD until Paper Publication
==Function==
[[http://www.uniprot.org/uniprot/AMIE_BACSP AMIE_BACSP]] Catalyzes the hydrolysis of short-chain aliphatic amides to their corresponding organic acids with release of ammonia.<ref>PMID:10978771</ref>  Also exhibits in vitro acyl transferase activity, transferring the acyl moiety of short-chain amides to hydroxylamine to form hydroxamates (By similarity).<ref>PMID:10978771</ref>  


Authors: Sewell, B.T., Weber, B.W., Kimani, S.W., Cowan, D.A., Hunter, R., Venter, G.A., Gumbart, J.C., Thuku, R.N., Varsani, A.
==About this Structure==
[[4lf0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp. Bacillus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LF0 OCA].  


Description: The E142D mutant of the amidase from Geobacillus pallidus
==Reference==
<references group="xtra"/><references/>
[[Category: Amidase]]
[[Category: Bacillus sp.]]
[[Category: Cowan, D A.]]
[[Category: Gumbart, J C.]]
[[Category: Hunter, R.]]
[[Category: Kimani, S W.]]
[[Category: Sewell, B T.]]
[[Category: Thuku, R N.]]
[[Category: Varsani, A.]]
[[Category: Venter, G A.]]
[[Category: Weber, B W.]]
[[Category: Alpha-beta-beta-alpha sandwich]]
[[Category: Hydrolase]]

Revision as of 08:37, 21 August 2013

Template:STRUCTURE 4lf0

The E142D mutant of the amidase from Geobacillus pallidus

Function

[AMIE_BACSP] Catalyzes the hydrolysis of short-chain aliphatic amides to their corresponding organic acids with release of ammonia.[1] Also exhibits in vitro acyl transferase activity, transferring the acyl moiety of short-chain amides to hydroxylamine to form hydroxamates (By similarity).[2]

About this Structure

4lf0 is a 1 chain structure with sequence from Bacillus sp.. Full crystallographic information is available from OCA.

Reference

  1. ↑ Kim S, Oriel P. Cloning and expression of the nitrile hydratase and amidase genes from Bacillus sp. BR449 into Escherichia coli. Enzyme Microb Technol. 2000 Oct 1;27(7):492-501. PMID:10978771
  2. ↑ Kim S, Oriel P. Cloning and expression of the nitrile hydratase and amidase genes from Bacillus sp. BR449 into Escherichia coli. Enzyme Microb Technol. 2000 Oct 1;27(7):492-501. PMID:10978771

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