3s2u: Difference between revisions
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==Crystal structure of the Pseudomonas aeruginosa MurG:UDP-GlcNAc substrate complex== | |||
=== | <StructureSection load='3s2u' size='340' side='right' caption='[[3s2u]], [[Resolution|resolution]] 2.23Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3s2u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S2U FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">murG, PA4412 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 Pseudomonas aeruginosa PAO1])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Undecaprenyldiphospho-muramoylpentapeptide_beta-N-_acetylglucosaminyltransferase Undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.227 2.4.1.227] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s2u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s2u RCSB], [http://www.ebi.ac.uk/pdbsum/3s2u PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
MurG is an essential bacterial glycosyltransferase enzyme in Pseudomonas aeruginosa performing one of the key membrane steps of peptidoglycan synthesis catalyzing the transfer of N-acetyl glucosamine (GlcNAc) from its donor substrate, UDP-GlcNAc, to the acceptor substrate Lipid I. We have solved the crystal structure of the complex between Pseudomonas aeruginosa MurG and UDP-GlcNAc and compared it with the previously solved complex from E. coli. The structure reveals a large-scale conformational change in the relative orientations of the N- and C-terminal domains, which has the effect of widening the cofactor binding site and displacing the UDP-GlcNAc donor. These results suggest new opportunities to design potent inhibitors of peptidoglycan biosynthesis. | |||
Crystal structure of the Pseudomonas aeruginosa MurG:UDP-GlcNAc substrate complex.,Brown K, Vial SC, Dedi N, Westcott J, Scally S, Bugg TD, Charlton PA, Cheetham GM Protein Pept Lett. 2012 Sep 4. PMID:22973843<ref>PMID:22973843</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Pseudomonas aeruginosa pao1]] | [[Category: Pseudomonas aeruginosa pao1]] | ||
[[Category: Undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase]] | [[Category: Undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase]] | ||
[[Category: Brown, K | [[Category: Brown, K]] | ||
[[Category: Bugg, T D.H | [[Category: Bugg, T D.H]] | ||
[[Category: Charlton, P A | [[Category: Charlton, P A]] | ||
[[Category: Cheetham, G M.T | [[Category: Cheetham, G M.T]] | ||
[[Category: Dedi, N | [[Category: Dedi, N]] | ||
[[Category: Scally, S | [[Category: Scally, S]] | ||
[[Category: Vial, S C.M | [[Category: Vial, S C.M]] | ||
[[Category: Westcott, J | [[Category: Westcott, J]] | ||
[[Category: N-acetylglucosaminyl transferase]] | [[Category: N-acetylglucosaminyl transferase]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
