2jm3: Difference between revisions
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[[Image:2jm3.jpg|left|200px]]< | [[Image:2jm3.jpg|left|200px]] | ||
'''Solution structure of the THAP domain from C. elegans C-terminal binding protein (CtBP)''' | {{Structure | ||
|PDB= 2jm3 |SIZE=350|CAPTION= <scene name='initialview01'>2jm3</scene> | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |||
|ACTIVITY= | |||
|GENE= F49E10.5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans]) | |||
}} | |||
'''Solution structure of the THAP domain from C. elegans C-terminal binding protein (CtBP)''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2JM3 is a [ | 2JM3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JM3 OCA]. | ||
==Reference== | ==Reference== | ||
Solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP)., Liew CK, Crossley M, Mackay JP, Nicholas HR, J Mol Biol. 2007 Feb 16;366(2):382-90. Epub 2006 Nov 18. PMID:[http:// | Solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP)., Liew CK, Crossley M, Mackay JP, Nicholas HR, J Mol Biol. 2007 Feb 16;366(2):382-90. Epub 2006 Nov 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17174978 17174978] | ||
[[Category: Caenorhabditis elegans]] | [[Category: Caenorhabditis elegans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: zinc finger]] | [[Category: zinc finger]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:42:42 2008'' | ||
Revision as of 15:42, 20 March 2008
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| 2jm3 | |||||||||||||
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| Ligands: | ZN | ||||||||||||
| Gene: | F49E10.5 (Caenorhabditis elegans) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Solution structure of the THAP domain from C. elegans C-terminal binding protein (CtBP)
Overview
The THAP (Thanatos-associated protein) domain is a recently discovered zinc-binding domain found in proteins involved in transcriptional regulation, cell-cycle control, apoptosis and chromatin modification. It contains a single zinc atom ligated by cysteine and histidine residues within a Cys-X(2-4)-Cys-X(35-53)-Cys-X(2)-His consensus. We have determined the NMR solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP) and show that it adopts a fold containing a treble clef motif, bearing similarity to the zinc finger-associated domain (ZAD) from Drosophila Grauzone. The CtBP THAP domain contains a large, positively charged surface patch and we demonstrate that this domain can bind to double-stranded DNA in an electrophoretic mobility-shift assay. These data, together with existing reports, indicate that THAP domains might exhibit a functional diversity similar to that observed for classical and GATA-type zinc fingers.
About this Structure
2JM3 is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
Solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP)., Liew CK, Crossley M, Mackay JP, Nicholas HR, J Mol Biol. 2007 Feb 16;366(2):382-90. Epub 2006 Nov 18. PMID:17174978
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