2jmh: Difference between revisions
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'''NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis''' | {{Structure | ||
|PDB= 2jmh |SIZE=350|CAPTION= <scene name='initialview01'>2jmh</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= BLOT5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Blomia tropicalis]) | |||
}} | |||
'''NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2JMH is a [ | 2JMH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Blomia_tropicalis Blomia tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMH OCA]. | ||
==Reference== | ==Reference== | ||
Roles of structure and structural dynamics in the antibody recognition of the allergen proteins: an NMR study on Blomia tropicalis major allergen., Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH, Structure. 2008 Jan;16(1):125-36. PMID:[http:// | Roles of structure and structural dynamics in the antibody recognition of the allergen proteins: an NMR study on Blomia tropicalis major allergen., Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH, Structure. 2008 Jan;16(1):125-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18184590 18184590] | ||
[[Category: Blomia tropicalis]] | [[Category: Blomia tropicalis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: allergen]] | [[Category: allergen]] | ||
[[Category: blo t 5]] | [[Category: blo t 5]] | ||
[[Category: blomia | [[Category: blomia tropicali]] | ||
[[Category: dust | [[Category: dust mite]] | ||
[[Category: group 5]] | [[Category: group 5]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:42:52 2008'' | ||
Revision as of 15:42, 20 March 2008
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| Gene: | BLOT5 (Blomia tropicalis) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis
Overview
Blo t 5 is the major allergen from Blomia tropicalis mites and shows strong IgE reactivity with up to 90% of asthmatic and rhinitis patients' sera. The NMR solution structure of Blo t 5 comprises three long alpha helices, forming a coiled-coil, triple-helical bundle with a left-handed twist. TROSY-NMR experiments were used to study Blo t 5 interaction with the Fab' of a specific monoclonal antibody, mAb 4A7. The mAb epitope comprises two closely spaced surfaces, I and II, connected by a sharp turn and bearing critical residues Asn46 and Lys47 on one surface, and Lys54 and Arg57 on the other. This discontinuous epitope overlaps with the human IgE epitope(s) of Blo t 5. Epitope surface II is further predicted to undergo conformational exchange in the millisecond to microsecond range. The results presented are critical for the design of a hypoallergenic Blo t 5 for efficacious immunotherapy of allergic diseases.
About this Structure
2JMH is a Single protein structure of sequence from Blomia tropicalis. Full crystallographic information is available from OCA.
Reference
Roles of structure and structural dynamics in the antibody recognition of the allergen proteins: an NMR study on Blomia tropicalis major allergen., Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH, Structure. 2008 Jan;16(1):125-36. PMID:18184590
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