4k7h: Difference between revisions
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==Major capsid protein P1 of the Pseudomonas phage phi6== | |||
=== | <StructureSection load='4k7h' size='340' side='right' caption='[[4k7h]], [[Resolution|resolution]] 3.60Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4k7h]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K7H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K7H FirstGlance]. <br> | |||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">P1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10879 Pseudomonas phage phi6])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k7h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k7h RCSB], [http://www.ebi.ac.uk/pdbsum/4k7h PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The cystovirus varphi6 shares several distinct features with other double-stranded RNA (dsRNA) viruses, including the human pathogen, rotavirus: segmented genomes, nonequivalent packing of 120 subunits in its icosahedral capsid, and capsids as compartments for transcription and replication. varphi6 assembles as a dodecahedral procapsid that undergoes major conformational changes as it matures into the spherical capsid. We determined the crystal structure of the capsid protein, P1, revealing a flattened trapezoid subunit with an alpha-helical fold. We also solved the procapsid with cryo-electron microscopy to comparable resolution. Fitting the crystal structure into the procapsid disclosed substantial conformational differences between the two P1 conformers. Maturation via two intermediate states involves remodeling on a similar scale, besides huge rigid-body rotations. The capsid structure and its stepwise maturation that is coupled to sequential packaging of three RNA segments sets the cystoviruses apart from other dsRNA viruses as a dynamic molecular machine. | |||
Subunit Folds and Maturation Pathway of a dsRNA Virus Capsid.,Nemecek D, Boura E, Wu W, Cheng N, Plevka P, Qiao J, Mindich L, Heymann JB, Hurley JH, Steven AC Structure. 2013 Aug 6;21(8):1374-83. doi: 10.1016/j.str.2013.06.007. Epub 2013, Jul 25. PMID:23891288<ref>PMID:23891288</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
== | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Pseudomonas phage phi6]] | [[Category: Pseudomonas phage phi6]] | ||
[[Category: Boura, E | [[Category: Boura, E]] | ||
[[Category: Hurley, J H | [[Category: Hurley, J H]] | ||
[[Category: Nemecek, D | [[Category: Nemecek, D]] | ||
[[Category: Plevka, P | [[Category: Plevka, P]] | ||
[[Category: Steven, C A | [[Category: Steven, C A]] | ||
[[Category: Major capsid protein]] | [[Category: Major capsid protein]] | ||
[[Category: Viral protein]] | [[Category: Viral protein]] | ||
Revision as of 15:14, 21 December 2014
Major capsid protein P1 of the Pseudomonas phage phi6
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