2jpz: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2jpz.gif|left|200px]] | [[Image:2jpz.gif|left|200px]] | ||
'''Structure of the hybrid-2 type intramolecular human telomeric G-quadruplex''' | {{Structure | ||
|PDB= 2jpz |SIZE=350|CAPTION= <scene name='initialview01'>2jpz</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Structure of the hybrid-2 type intramolecular human telomeric G-quadruplex''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
2JPZ is a [ | 2JPZ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JPZ OCA]. | ||
==Reference== | ==Reference== | ||
Structure of the Hybrid-2 type intramolecular human telomeric G-quadruplex in K+ solution: insights into structure polymorphism of the human telomeric sequence., Dai J, Carver M, Punchihewa C, Jones RA, Yang D, Nucleic Acids Res. 2007;35(15):4927-40. Epub 2007 Jul 10. PMID:[http:// | Structure of the Hybrid-2 type intramolecular human telomeric G-quadruplex in K+ solution: insights into structure polymorphism of the human telomeric sequence., Dai J, Carver M, Punchihewa C, Jones RA, Yang D, Nucleic Acids Res. 2007;35(15):4927-40. Epub 2007 Jul 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17626043 17626043] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Carver, M.]] | [[Category: Carver, M.]] | ||
| Line 22: | Line 31: | ||
[[Category: quadruplex]] | [[Category: quadruplex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:44:07 2008'' | ||
Revision as of 15:44, 20 March 2008
| |||||||||||||
| 2jpz | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Structure of the hybrid-2 type intramolecular human telomeric G-quadruplex
Overview
Formation of the G-quadruplex in the human telomeric sequence can inhibit the activity of telomerase, thus the intramolecular telomeric G-quadruplexes have been considered as an attractive anticancer target. Information of intramolecular telomeric G-quadruplex structures formed under physiological conditions is important for structure-based drug design. Here, we report the first structure of the major intramolecular G-quadruplex formed in a native, non-modified human telomeric sequence in K(+) solution. This is a hybrid-type mixed parallel/antiparallel-G-stranded G-quadruplex, one end of which is covered by a novel T:A:T triple capping structure. This structure (Hybrid-2) and the previously reported Hybrid-1 structure differ in their loop arrangements, strand orientations and capping structures. The distinct capping structures appear to be crucial for the favored formation of the specific hybrid-type intramolecular telomeric G-quadruplexes, and may provide specific binding sites for drug targeting. Our study also shows that while the hybrid-type G-quadruplexes appear to be the major conformations in K(+) solution, human telomeric sequences are always in equilibrium between Hybrid-1 and Hybrid-2 structures, which is largely determined by the 3'-flanking sequence. Furthermore, both hybrid-type G-quadruplexes suggest a straightforward means for multimer formation with effective packing in the human telomeric sequence and provide important implications for drug targeting of G-quadruplexes in human telomeres.
About this Structure
2JPZ is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of the Hybrid-2 type intramolecular human telomeric G-quadruplex in K+ solution: insights into structure polymorphism of the human telomeric sequence., Dai J, Carver M, Punchihewa C, Jones RA, Yang D, Nucleic Acids Res. 2007;35(15):4927-40. Epub 2007 Jul 10. PMID:17626043
Page seeded by OCA on Thu Mar 20 17:44:07 2008
