3j45: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

===Structure of a non-translocating SecY protein channel with the 70S ribosome===

The ~~entry 3j45~~ is ~~ON HOLD~~ ~~until Paper Publication~~

==Function==

[[http://www.uniprot.org/uniprot/RL29_ECOLI RL29_ECOLI]] Binds 23S rRNA. It is not essential for growth.[HAMAP-Rule:MF_00374] One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit. Contacts trigger factor (PubMed:12226666).[HAMAP-Rule:MF_00374] [[http://www.uniprot.org/uniprot/SECG_ECOLI SECG_ECOLI]] Subunit of the protein translocation channel SecYEG. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true it also results in FtsH-mediated degradation of SecY. Treatment with antibiotics that block translation elongation such as chloramphenicol also leads to degradation of SecY and SecE but not SecG. [[http://www.uniprot.org/uniprot/SECE_ECOLI SECE_ECOLI]] Essential subunit of the protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true it also results in FtsH-mediated degradation of SecY.<ref>PMID:15140892</ref> [[http://www.uniprot.org/uniprot/SECY_ECOLI SECY_ECOLI]] The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently. SecY is required to insert newly synthesized SecY into the inner membrane. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true, overexpression also results in FtsH-mediated degradation of SecY.[HAMAP-Rule:MF_01465] [[http://www.uniprot.org/uniprot/RL23_ECOLI RL23_ECOLI]] One of the early assembly proteins, it binds 23S rRNA; is essential for growth. One of the proteins that surround the polypeptide exit tunnel on the outside of the subunit. Acts as the docking site for trigger factor (PubMed:12226666) for Ffh binding to the ribosome (SRP54, PubMed:12756233 and PubMed:12702815) and to nascent polypeptide chains (PubMed:12756233).[HAMAP-Rule:MF_01369] [[http://www.uniprot.org/uniprot/RL24_ECOLI RL24_ECOLI]] One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit. It is not thought to be involved in the functions of the mature 50S subunit in vitro.<ref>PMID:357435</ref> One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit.<ref>PMID:357435</ref>

~~Authors~~: ~~Menetret, J~~.F., ~~Park, E~~.~~, Gumbart, J~~.C.~~, Ludtke, S~~.J.~~, Li, W~~.~~, Whynot, A~~.~~, Rapoport, T.A., Akey, C~~.W.

==About this Structure==

[[3j45]] is a 11 chain structure with sequence from [http://en.wikipedia.org/wiki/Cortinarius_roseobulbus Cortinarius roseobulbus], [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Gekko_coi Gekko coi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J45 OCA].

~~Description~~: ~~Structure of a non~~-~~translocating SecY~~ protein ~~channel with the 70S ribosome~~

==Reference==

[[Category: Cortinarius roseobulbus]]

[[Category: Escherichia coli]]

[[Category: Gekko coi]]

[[Category: Akey, C W.]]

[[Category: Gumbart, J C.]]

[[Category: Li, W.]]

[[Category: Ludtke, S J.]]

[[Category: Menetret, J F.]]

[[Category: Park, E.]]

[[Category: Rapoport, T A.]]

[[Category: Whynot, A.]]

[[Category: 70]]

[[Category: Protein translocation channel]]

[[Category: Ribosome-protein transport complex]]

[[Category: Secyeg]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+{{STRUCTURE_3j45|  PDB=3j45  |  SCENE=  }}
+===Structure of a non-translocating SecY protein channel with the 70S ribosome===
-The entry 3j45 is ON HOLD  until Paper Publication
+==Function==
+[[http://www.uniprot.org/uniprot/RL29_ECOLI RL29_ECOLI]] Binds 23S rRNA. It is not essential for growth.[HAMAP-Rule:MF_00374]  One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit. Contacts trigger factor (PubMed:12226666).[HAMAP-Rule:MF_00374] [[http://www.uniprot.org/uniprot/SECG_ECOLI SECG_ECOLI]] Subunit of the protein translocation channel SecYEG. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true it also results in FtsH-mediated degradation of SecY. Treatment with antibiotics that block translation elongation such as chloramphenicol also leads to degradation of SecY and SecE but not SecG. [[http://www.uniprot.org/uniprot/SECE_ECOLI SECE_ECOLI]] Essential subunit of the protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true it also results in FtsH-mediated degradation of SecY.<ref>PMID:15140892</ref>  [[http://www.uniprot.org/uniprot/SECY_ECOLI SECY_ECOLI]] The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently. SecY is required to insert newly synthesized SecY into the inner membrane. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true, overexpression also results in FtsH-mediated degradation of SecY.[HAMAP-Rule:MF_01465] [[http://www.uniprot.org/uniprot/RL23_ECOLI RL23_ECOLI]] One of the early assembly proteins, it binds 23S rRNA; is essential for growth. One of the proteins that surround the polypeptide exit tunnel on the outside of the subunit. Acts as the docking site for trigger factor (PubMed:12226666) for Ffh binding to the ribosome (SRP54, PubMed:12756233 and PubMed:12702815) and to nascent polypeptide chains (PubMed:12756233).[HAMAP-Rule:MF_01369] [[http://www.uniprot.org/uniprot/RL24_ECOLI RL24_ECOLI]] One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit. It is not thought to be involved in the functions of the mature 50S subunit in vitro.<ref>PMID:357435</ref>   One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit.<ref>PMID:357435</ref>
-Authors: Menetret, J.F., Park, E., Gumbart, J.C., Ludtke, S.J., Li, W., Whynot, A., Rapoport, T.A., Akey, C.W.
+==About this Structure==
+[[3j45]] is a 11 chain structure with sequence from [http://en.wikipedia.org/wiki/Cortinarius_roseobulbus Cortinarius roseobulbus], [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Gekko_coi Gekko coi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J45 OCA].
-Description: Structure of a non-translocating SecY protein channel with the 70S ribosome
+==Reference==
+<references group="xtra"/><references/>
+[[Category: Cortinarius roseobulbus]]
+[[Category: Escherichia coli]]
+[[Category: Gekko coi]]
+[[Category: Akey, C W.]]
+[[Category: Gumbart, J C.]]
+[[Category: Li, W.]]
+[[Category: Ludtke, S J.]]
+[[Category: Menetret, J F.]]
+[[Category: Park, E.]]
+[[Category: Rapoport, T A.]]
+[[Category: Whynot, A.]]
+[[Category: 70]]
+[[Category: Protein translocation channel]]
+[[Category: Ribosome-protein transport complex]]
+[[Category: Secyeg]]