4gyn: Difference between revisions
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{{STRUCTURE_4gyn| PDB=4gyn | SCENE= }} | {{STRUCTURE_4gyn| PDB=4gyn | SCENE= }} | ||
===The E142L mutant of the amidase from Geobacillus pallidus=== | ===The E142L mutant of the amidase from Geobacillus pallidus=== | ||
{{ABSTRACT_PUBMED_23946488}} | |||
==Function== | ==Function== | ||
| Line 6: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
[[4gyn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/ | [[4gyn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsp Bacsp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GYN OCA]. | ||
==Reference== | ==Reference== | ||
<references group="xtra"/><references/> | <ref group="xtra">PMID:023946488</ref><references group="xtra"/><references/> | ||
[[Category: Amidase]] | [[Category: Amidase]] | ||
[[Category: | [[Category: Bacsp]] | ||
[[Category: Cowan, D A.]] | [[Category: Cowan, D A.]] | ||
[[Category: Hunter, R.]] | [[Category: Hunter, R.]] | ||
Revision as of 08:56, 5 February 2014
The E142L mutant of the amidase from Geobacillus pallidus
Template:ABSTRACT PUBMED 23946488
Function
[AMIE_BACSP] Catalyzes the hydrolysis of short-chain aliphatic amides to their corresponding organic acids with release of ammonia.[1] Also exhibits in vitro acyl transferase activity, transferring the acyl moiety of short-chain amides to hydroxylamine to form hydroxamates (By similarity).[2]
About this Structure
4gyn is a 1 chain structure with sequence from Bacsp. Full crystallographic information is available from OCA.
Reference
- Weber BW, Kimani SW, Varsani A, Cowan DA, Hunter R, Venter GA, Gumbart JC, Sewell BT. The mechanism of the amidases: mutating the glutamate adjacent to the catalytic triad inactivates the enzyme due to substrate mispositioning. J Biol Chem. 2013 Oct 4;288(40):28514-23. doi: 10.1074/jbc.M113.503284. Epub 2013, Aug 14. PMID:23946488 doi:https://dx.doi.org/10.1074/jbc.M113.503284
- ↑ Kim S, Oriel P. Cloning and expression of the nitrile hydratase and amidase genes from Bacillus sp. BR449 into Escherichia coli. Enzyme Microb Technol. 2000 Oct 1;27(7):492-501. PMID:10978771
- ↑ Kim S, Oriel P. Cloning and expression of the nitrile hydratase and amidase genes from Bacillus sp. BR449 into Escherichia coli. Enzyme Microb Technol. 2000 Oct 1;27(7):492-501. PMID:10978771
