4e2i: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
Line 1: Line 1:
{{STRUCTURE_4e2i|  PDB=4e2i  |  SCENE=  }}
==The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase==
===The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase===
<StructureSection load='4e2i' size='340' side='right' caption='[[4e2i]], [[Resolution|resolution]] 5.00&Aring;' scene=''>
{{ABSTRACT_PUBMED_22700977}}
== Structural highlights ==
<table><tr><td colspan='2'>[[4e2i]] is a 23 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E2I FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">POLA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e2i RCSB], [http://www.ebi.ac.uk/pdbsum/4e2i PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/DPOA2_HUMAN DPOA2_HUMAN]] May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
DNA polymerase alpha-primase (Pol-prim) plays an essential role in eukaryotic DNA replication, initiating synthesis of the leading strand and of each Okazaki fragment on the lagging strand. Pol-prim is composed of a primase heterodimer that synthesizes an RNA primer, a DNA polymerase subunit that extends the primer, and a regulatory B-subunit (p68) without apparent enzymatic activity. Pol-prim is thought to interact with eukaryotic replicative helicases, forming a dynamic multiprotein assembly that displays primosome activity. At least three subunits of Pol-prim interact physically with the hexameric replicative helicase SV40 large T antigen, constituting a simple primosome that is active in vitro. However, structural understanding of these interactions and their role in viral chromatin replication in vivo remains incomplete. Here, we report the detailed large T antigen-p68 interface, as revealed in a co-crystal structure and validated by site-directed mutagenesis, and we demonstrate its functional importance in activating the SV40 primosome in cell-free reactions with purified Pol-prim, as well as in monkey cells in vivo.


==Function==
Structural basis for the interaction of a hexameric replicative helicase with the regulatory subunit of human DNA polymerase alpha-primase.,Zhou B, Arnett DR, Yu X, Brewster A, Sowd GA, Xie CL, Vila S, Gai D, Fanning E, Chen XS J Biol Chem. 2012 Aug 3;287(32):26854-66. doi: 10.1074/jbc.M112.363655. Epub 2012, Jun 14. PMID:22700977<ref>PMID:22700977</ref>
[[http://www.uniprot.org/uniprot/DPOA2_HUMAN DPOA2_HUMAN]] May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity).  


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[4e2i]] is a 23 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2I OCA].
</div>


==See Also==
==See Also==
*[[DNA polymerase|DNA polymerase]]
*[[DNA polymerase|DNA polymerase]]
 
*[[Large T Antigen|Large T Antigen]]
==Reference==
== References ==
<ref group="xtra">PMID:022700977</ref><references group="xtra"/><references/>
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Simian virus 40]]
[[Category: Simian virus 40]]
[[Category: Arnett, D R.]]
[[Category: Arnett, D R]]
[[Category: Brewster, A.]]
[[Category: Brewster, A]]
[[Category: Chen, X S.]]
[[Category: Chen, X S]]
[[Category: Fanning, E.]]
[[Category: Fanning, E]]
[[Category: Gai, D.]]
[[Category: Gai, D]]
[[Category: Sowd, G A.]]
[[Category: Sowd, G A]]
[[Category: Vila, S.]]
[[Category: Vila, S]]
[[Category: Xie, C L.]]
[[Category: Xie, C L]]
[[Category: Yu, X.]]
[[Category: Yu, X]]
[[Category: Zhou, B.]]
[[Category: Zhou, B]]
[[Category: Hydrolase-dna binding complex]]
[[Category: Hydrolase-dna binding complex]]
[[Category: Hydrolase-dna binding protein complex]]
[[Category: Hydrolase-dna binding protein complex]]
[[Category: Replication initiation]]
[[Category: Replication initiation]]
Retrieved from "https://proteopedia.org/4e2i"