2ymt: Difference between revisions
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==gamma 2 adaptin EAR domain crystal structure with phage peptide GEEWGPWV== | |||
<StructureSection load='2ymt' size='340' side='right' caption='[[2ymt]], [[Resolution|resolution]] 1.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2ymt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YMT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YMT FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PDO:1,3-PROPANDIOL'>PDO</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bcx|4bcx]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ymt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ymt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ymt RCSB], [http://www.ebi.ac.uk/pdbsum/2ymt PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Hepatitis B virus (HBV) is an infectious, potentially lethal human pathogen. However, there are no effective therapies for chronic HBV infections. Antiviral development is hampered by the lack of high-resolution structures for essential HBV protein-protein interactions. The interaction between preS1, an HBV surface-protein domain, and its human binding partner, gamma2-adaptin, subverts the membrane-trafficking apparatus to mediate virion export. This interaction is a putative drug target. We report here atomic-resolution descriptions of the binding thermodynamics and structural biology of the interaction between preS1 and the EAR domain of gamma2-adaptin. NMR, protein engineering, X-ray crystallography and MS showed that preS1 contains multiple gamma2-EAR-binding motifs that mimic the membrane-trafficking motifs (and binding modes) of host proteins. These motifs localize together to a relatively rigid, functionally important region of preS1, an intrinsically disordered protein. The preS1-gamma2-EAR interaction was relatively weak and efficiently outcompeted by a synthetic peptide. Our data provide the structural road map for developing peptidomimetic antivirals targeting the gamma2-EAR-preS1 interaction. | |||
The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry.,Jurgens MC, Voros J, Rautureau GJ, Shepherd DA, Pye VE, Muldoon J, Johnson CM, Ashcroft AE, Freund SM, Ferguson N Nat Chem Biol. 2013 Jul 14. doi: 10.1038/nchembio.1294. PMID:23851574<ref>PMID:23851574</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Adaptin|Adaptin]] | *[[Adaptin|Adaptin]] | ||
== References == | |||
== | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Ashcroft, A | [[Category: Ashcroft, A]] | ||
[[Category: Ferguson, N | [[Category: Ferguson, N]] | ||
[[Category: Freund, S M.V | [[Category: Freund, S M.V]] | ||
[[Category: Johnson, C M | [[Category: Johnson, C M]] | ||
[[Category: Juergens, M C | [[Category: Juergens, M C]] | ||
[[Category: Muldoon, J | [[Category: Muldoon, J]] | ||
[[Category: Pye, V E | [[Category: Pye, V E]] | ||
[[Category: Rautureau, G | [[Category: Rautureau, G]] | ||
[[Category: Shepherd, D | [[Category: Shepherd, D]] | ||
[[Category: Voros, J | [[Category: Voros, J]] | ||
[[Category: Protein transport]] | [[Category: Protein transport]] | ||
Revision as of 13:34, 18 December 2014
gamma 2 adaptin EAR domain crystal structure with phage peptide GEEWGPWV
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