4ki2: Difference between revisions
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==Crystallographic analysis of an RNA-polymerase sigma-subunit fragment complexed with -10 promoter element ssDNA== | |||
<StructureSection load='4ki2' size='340' side='right' caption='[[4ki2]], [[Resolution|resolution]] 3.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4ki2]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25104 Atcc 25104]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4gor 4gor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KI2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KI2 FirstGlance]. <br> | |||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene><br> | |||
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ugo|3ugo]], [[3ugp|3ugp]]</td></tr> | |||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ki2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ki2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ki2 RCSB], [http://www.ebi.ac.uk/pdbsum/4ki2 PDBsum]</span></td></tr> | |||
<table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Structural studies of -10 promoter element recognition by domain 2 of the RNA polymerase sigma subunit [Feklistov & Darst (2011), Cell, 147, 1257-1269] reveal an unusual crystal-packing arrangement dominated by G-quartets. The 3'-terminal GGG motif of the oligonucleotide used in crystallization participates in G-quadruplex formation with GGG motifs from symmetry-related complexes. Stacking between neighboring G-quadruplexes results in the formation of pseudo-continuous four-stranded columns running throughout the length of the crystal (G-columns). Here, a new crystal form is presented with a different arrangement of G-columns and it is proposed that the fortuitous finding of G-quartet packing could be useful in engineering crystal contacts in protein-ssDNA complexes. | |||
Crystallographic analysis of an RNA polymerase sigma-subunit fragment complexed with -10 promoter element ssDNA: quadruplex formation as a possible tool for engineering crystal contacts in protein-ssDNA complexes.,Feklistov A, Darst SA Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Sep;69(Pt 9):950-5. doi:, 10.1107/S1744309113020368. Epub 2013 Aug 19. PMID:23989139<ref>PMID:23989139</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
[[ | </div> | ||
[[Category: | |||
==See Also== | |||
*[[Sigma factor|Sigma factor]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Atcc 25104]] | |||
[[Category: Feklistov, A.]] | [[Category: Feklistov, A.]] | ||
[[Category: S A., Darst.]] | [[Category: S A., Darst.]] | ||
Revision as of 05:40, 24 September 2014
Crystallographic analysis of an RNA-polymerase sigma-subunit fragment complexed with -10 promoter element ssDNA
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