2o5n: Difference between revisions
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'''Crystal structure of a Viral Glycoprotein''' | {{Structure | ||
|PDB= 2o5n |SIZE=350|CAPTION= <scene name='initialview01'>2o5n</scene>, resolution 2.400Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene> | |||
|ACTIVITY= | |||
|GENE= MuHV1gpm153 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10366 Murid herpesvirus 1]) | |||
}} | |||
'''Crystal structure of a Viral Glycoprotein''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2O5N is a [ | 2O5N is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Murid_herpesvirus_1 Murid herpesvirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O5N OCA]. | ||
==Reference== | ==Reference== | ||
Cellular expression and crystal structure of the murine cytomegalovirus major histocompatibility complex class I-like glycoprotein, m153., Mans J, Natarajan K, Balbo A, Schuck P, Eikel D, Hess S, Robinson H, Simic H, Jonjic S, Tiemessen CT, Margulies DH, J Biol Chem. 2007 Nov 30;282(48):35247-58. Epub 2007 Sep 26. PMID:[http:// | Cellular expression and crystal structure of the murine cytomegalovirus major histocompatibility complex class I-like glycoprotein, m153., Mans J, Natarajan K, Balbo A, Schuck P, Eikel D, Hess S, Robinson H, Simic H, Jonjic S, Tiemessen CT, Margulies DH, J Biol Chem. 2007 Nov 30;282(48):35247-58. Epub 2007 Sep 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17897947 17897947] | ||
[[Category: Murid herpesvirus 1]] | [[Category: Murid herpesvirus 1]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: viral protein]] | [[Category: viral protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:55:22 2008'' | ||
Revision as of 15:55, 20 March 2008
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| 2o5n, resolution 2.400Å | |||||||||||||
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| Ligands: | NDG | ||||||||||||
| Gene: | MuHV1gpm153 (Murid herpesvirus 1) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of a Viral Glycoprotein
Overview
Mouse cytomegalovirus (MCMV), a beta-herpesvirus that establishes latent and persistent infections in mice, is a valuable model for studying complex virus-host interactions. MCMV encodes the m145 family of putative immunoevasins with predicted major histocompatibility complex, class I (MHC-I) structure. Functions attributed to some family members include down-regulation of host MHC-I (m152) and NKG2D ligands (m145, m152, and m155) and interaction with inhibitory or activating NK receptors (m157). We present the cellular, biochemical, and structural characterization of m153, which is a heavily glycosylated homodimer, that does not require beta2m or peptide and is expressed at the surface of MCMV-infected cells. Its 2.4-A crystal structure confirms that this compact molecule preserves an MHC-I-like fold and reveals a novel mode of dimerization, confirmed by site-directed mutagenesis, and a distinctive disulfide-stabilized extended N terminus. The structure provides a useful framework for comparative analysis of the divergent members of the m145 family.
About this Structure
2O5N is a Protein complex structure of sequences from Murid herpesvirus 1. Full crystallographic information is available from OCA.
Reference
Cellular expression and crystal structure of the murine cytomegalovirus major histocompatibility complex class I-like glycoprotein, m153., Mans J, Natarajan K, Balbo A, Schuck P, Eikel D, Hess S, Robinson H, Simic H, Jonjic S, Tiemessen CT, Margulies DH, J Biol Chem. 2007 Nov 30;282(48):35247-58. Epub 2007 Sep 26. PMID:17897947
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