4idx: Difference between revisions
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==hexameric crystal structure of Schmallenberg virus nucleoprotein== | |||
=== | <StructureSection load='4idx' size='340' side='right' caption='[[4idx]], [[Resolution|resolution]] 3.21Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4idx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Schmallenberg_virus Schmallenberg virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IDX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IDX FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4idu|4idu]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1133363 Schmallenberg virus])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4idx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4idx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4idx RCSB], [http://www.ebi.ac.uk/pdbsum/4idx PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Schmallenberg virus (SBV), a newly emerged orthobunyavirus (family Bunyaviridae), has spread rapidly across Europe, and has caused congenital abnormalities in the offspring of cattle, sheep and goats. Like other orthobunyaviruses, SBV contains a tripartite negative-sense RNA genome that encodes four structural and two nonstructural proteins. The nucleoprotein (N) encapsidates the three viral genomic RNA segments and plays a crucial role in viral RNA transcription and replication. Here we report the crystal structure of bacterially-expressed SBV nucleoprotein to 3.06A resolution. The protomer is composed of two domains (N-terminal and C-terminal domains) with flexible N-terminal and C-terminal arms. The N protein has a novel fold, and forms a central positively charged cleft for genomic RNA binding. The nucleoprotein purified under native conditions forms a tetramer, while the nucleoprotein obtained following denaturation and refolding forms a hexamer. Our structural and functional analyses demonstrate that both N-terminal and C-terminal arms are involved in N-N interaction and oligomerization and play an essential role in viral RNA synthesis, suggesting a novel mechanism for viral RNA encapsidation and transcription. | |||
Structure of Schmallenberg orthobunyavirus nucleoprotein suggests a novel mechanism for genome encapsidation.,Dong H, Li P, Elliott RM, Dong C J Virol. 2013 Mar 6. PMID:23468499<ref>PMID:23468499</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
==See Also== | |||
*[[Nucleoprotein|Nucleoprotein]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Schmallenberg virus]] | [[Category: Schmallenberg virus]] | ||
[[Category: Dong, C J | [[Category: Dong, C J]] | ||
[[Category: Dong, H H | [[Category: Dong, H H]] | ||
[[Category: Dna binding protein]] | [[Category: Dna binding protein]] | ||
[[Category: Nucleoprotein]] | [[Category: Nucleoprotein]] | ||
Revision as of 12:46, 21 December 2014
hexameric crystal structure of Schmallenberg virus nucleoprotein
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