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Nitrogenase is an enzyme (protein) that fixes nitrogen (N2) from the atmosphere into ammonia (NH3). This enzyme mainly found in certain bacteria and blue-green algae in which some are free living( Cyanobacteria) or symbiotic (Rhizobia) in nature.
Nitrogenase catalyzes the following reaction:
N2 + 8H+ + 16MgATP + 8e− → 2NH3 + H2 + 16MgADP + 16Pi