1w6s: Difference between revisions
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==Overview== | ==Overview== | ||
The crystal structure of methanol dehydrogenase (MDH) from, Methylobacterium extorquens has been refined without stereochemical, restraints at a resolution of 1.2 A. The high-resolution data have defined, the conformation of the tricyclic pyrroloquinoline quinone (PQQ) cofactor, ring as entirely planar. The detailed definition of the active-site, geometry has shown many features that are similar to the, quinohaemo-protein alcohol dehydrogenases from Comamonas testosteroni and, Pseudomonas putida, both of which possess MDH-like and cytochrome c-like, domains. Conserved features between the two types of PQQ-containing enzyme, suggest a common pathway for electron transfer between MDH and its, physiological electron acceptor cytochrome cL. A pathway for proton, transfer from the active site . | The crystal structure of methanol dehydrogenase (MDH) from, Methylobacterium extorquens has been refined without stereochemical, restraints at a resolution of 1.2 A. The high-resolution data have defined, the conformation of the tricyclic pyrroloquinoline quinone (PQQ) cofactor, ring as entirely planar. The detailed definition of the active-site, geometry has shown many features that are similar to the, quinohaemo-protein alcohol dehydrogenases from Comamonas testosteroni and, Pseudomonas putida, both of which possess MDH-like and cytochrome c-like, domains. Conserved features between the two types of PQQ-containing enzyme, suggest a common pathway for electron transfer between MDH and its, physiological electron acceptor cytochrome cL. A pathway for proton, transfer from the active site to the bulk solvent is also suggested. | ||
==About this Structure== | ==About this Structure== | ||
1W6S is a | 1W6S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Methylobacterium_extorquens Methylobacterium extorquens] with CA, PQQ and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(acceptor) Alcohol dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.99.8 1.1.99.8] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W6S OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: pqq]] | [[Category: pqq]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:00:34 2007'' | ||
