2pk9: Difference between revisions
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[[Image:2pk9.jpg|left|200px]] | [[Image:2pk9.jpg|left|200px]] | ||
'''Structure of the Pho85-Pho80 CDK-cyclin Complex of the Phosphate-responsive Signal Transduction Pathway''' | {{Structure | ||
|PDB= 2pk9 |SIZE=350|CAPTION= <scene name='initialview01'>2pk9</scene>, resolution 2.906Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC ACID'>MES</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Cyclin-dependent_kinase Cyclin-dependent kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.22 2.7.11.22] | |||
|GENE= PHO85, SSG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), PHO80, AGS3, TUP7, VAC5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |||
}} | |||
'''Structure of the Pho85-Pho80 CDK-cyclin Complex of the Phosphate-responsive Signal Transduction Pathway''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2PK9 is a [ | 2PK9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PK9 OCA]. | ||
==Reference== | ==Reference== | ||
Structure of the Pho85-Pho80 CDK-cyclin complex of the phosphate-responsive signal transduction pathway., Huang K, Ferrin-O'Connell I, Zhang W, Leonard GA, O'Shea EK, Quiocho FA, Mol Cell. 2007 Nov 30;28(4):614-23. PMID:[http:// | Structure of the Pho85-Pho80 CDK-cyclin complex of the phosphate-responsive signal transduction pathway., Huang K, Ferrin-O'Connell I, Zhang W, Leonard GA, O'Shea EK, Quiocho FA, Mol Cell. 2007 Nov 30;28(4):614-23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18042456 18042456] | ||
[[Category: Cyclin-dependent kinase]] | [[Category: Cyclin-dependent kinase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: transferase/cell cycle complex]] | [[Category: transferase/cell cycle complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:14:03 2008'' | ||
Revision as of 16:14, 20 March 2008
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| 2pk9, resolution 2.906Å | |||||||||||||
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| Ligands: | MES | ||||||||||||
| Gene: | PHO85, SSG3 (Saccharomyces cerevisiae), PHO80, AGS3, TUP7, VAC5 (Saccharomyces cerevisiae) | ||||||||||||
| Activity: | Cyclin-dependent kinase, with EC number 2.7.11.22 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Structure of the Pho85-Pho80 CDK-cyclin Complex of the Phosphate-responsive Signal Transduction Pathway
Overview
The ability to sense and respond appropriately to environmental changes is a primary requirement of all living organisms. In response to phosphate limitation, Saccharomyces cerevisiae induces transcription of a set of genes involved in the regulation of phosphate acquisition from the ambient environment. A signal transduction pathway (the PHO pathway) mediates this response, with Pho85-Pho80 playing a vital role. Here we report the X-ray structure of Pho85-Pho80, a prototypic structure of a CDK-cyclin complex functioning in transcriptional regulation in response to environmental changes. The structure revealed a specific salt link between a Pho85 arginine and a Pho80 aspartate that makes phosphorylation of the Pho85 activation loop dispensable and that maintains a Pho80 loop conformation for possible substrate recognition. It further showed two sites on the Pho80 cyclin for high-affinity binding of the transcription factor substrate (Pho4) and the CDK inhibitor (Pho81) that are markedly distant to each other and the active site.
About this Structure
2PK9 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of the Pho85-Pho80 CDK-cyclin complex of the phosphate-responsive signal transduction pathway., Huang K, Ferrin-O'Connell I, Zhang W, Leonard GA, O'Shea EK, Quiocho FA, Mol Cell. 2007 Nov 30;28(4):614-23. PMID:18042456
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