4hvy: Difference between revisions
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{{STRUCTURE_4hvy| PDB=4hvy | SCENE= }} | |||
===A thermostable variant of human NUDT18 NUDIX domain obtained by Hot Colony Filtration=== | |||
{{ABSTRACT_PUBMED_24352381}} | |||
==Function== | |||
[[http://www.uniprot.org/uniprot/NUD18_HUMAN NUD18_HUMAN]] Mediates the hydrolyzis of oxidized nucleoside diphosphate derivatives. Hydrolyzes 8-oxo-7,8-dihydroguanine (8-oxo-Gua)-containing deoxyribo- and ribonucleoside diphosphates to the monophosphates. Hydrolyzes 8-oxo-dGDP and 8-oxo-GDP with the same efficiencies. Hydrolyzes also 8-OH-dADP and 2-OH-dADP. Exhibited no or minimal hydrolyzis activity against 8-oxo-dGTP, 8-oxo-GTP, dGTP, GTP, dGDP and GDP. Probably removes oxidized guanine nucleotides from both the DNA and RNA precursor pools. | |||
==About this Structure== | |||
[[4hvy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HVY OCA]. | |||
==Reference== | |||
<ref group="xtra">PMID:024352381</ref><references group="xtra"/><references/> | |||
[[Category: Asial, I.]] | |||
[[Category: Cheng, Y X.]] | |||
[[Category: Cornvik, T.]] | |||
[[Category: Dollhopf, M.]] | |||
[[Category: Engman, H.]] | |||
[[Category: Nordlund, P.]] | |||
[[Category: Wu, B.]] | |||
[[Category: Directed evolution]] | |||
[[Category: Hot colony filtration]] | |||
[[Category: Hot-cofi]] | |||
[[Category: Hydrolase]] | |||
[[Category: Thermostability]] | |||
Revision as of 12:06, 1 January 2014
A thermostable variant of human NUDT18 NUDIX domain obtained by Hot Colony Filtration
Template:ABSTRACT PUBMED 24352381
Function
[NUD18_HUMAN] Mediates the hydrolyzis of oxidized nucleoside diphosphate derivatives. Hydrolyzes 8-oxo-7,8-dihydroguanine (8-oxo-Gua)-containing deoxyribo- and ribonucleoside diphosphates to the monophosphates. Hydrolyzes 8-oxo-dGDP and 8-oxo-GDP with the same efficiencies. Hydrolyzes also 8-OH-dADP and 2-OH-dADP. Exhibited no or minimal hydrolyzis activity against 8-oxo-dGTP, 8-oxo-GTP, dGTP, GTP, dGDP and GDP. Probably removes oxidized guanine nucleotides from both the DNA and RNA precursor pools.
About this Structure
4hvy is a 1 chain structure. Full crystallographic information is available from OCA.
Reference
- Asial I, Cheng YX, Engman H, Dollhopf M, Wu B, Nordlund P, Cornvik T. Engineering protein thermostability using a generic activity-independent biophysical screen inside the cell. Nat Commun. 2013 Dec 19;4:2901. doi: 10.1038/ncomms3901. PMID:24352381 doi:https://dx.doi.org/10.1038/ncomms3901
