1wb6: Difference between revisions
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==Overview== | ==Overview== | ||
Feruloyl esterases play a key role in the degradation of the intricate, structure of the plant cell wall by hydrolysing the ferulate ester groups, involved in the cross-linking between hemicelluloses and between, hemicellulose and lignin. The structure of the feruloyl esterase module of, Clostridium thermocellum cellulosomal xylanase 10B has been reported, previously. It displays the alpha/beta hydrolase fold with a classical, Ser-His-Asp catalytic triad. Here, the structures of a Ser-Ala mutant of, this feruloyl esterase in complexes with methyl syringate, methyl, sinapinate and methyl vanillate are described. Substrate binding is, accompanied by subtle conformational changes at amino acids Trp982, Met955, Asn1023 and Ile1019 in the ligand-binding cavity. The structural, determinants, . | Feruloyl esterases play a key role in the degradation of the intricate, structure of the plant cell wall by hydrolysing the ferulate ester groups, involved in the cross-linking between hemicelluloses and between, hemicellulose and lignin. The structure of the feruloyl esterase module of, Clostridium thermocellum cellulosomal xylanase 10B has been reported, previously. It displays the alpha/beta hydrolase fold with a classical, Ser-His-Asp catalytic triad. Here, the structures of a Ser-Ala mutant of, this feruloyl esterase in complexes with methyl syringate, methyl, sinapinate and methyl vanillate are described. Substrate binding is, accompanied by subtle conformational changes at amino acids Trp982, Met955, Asn1023 and Ile1019 in the ligand-binding cavity. The structural, determinants, particularly the m-methoxy substituent, governing the, substrate specificity of Xyn10B feruloyl esterase are rationalized. | ||
==About this Structure== | ==About this Structure== | ||
1WB6 is a | 1WB6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum] with ACT, CD, VXX and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WB6 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: xylanase]] | [[Category: xylanase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:49:16 2007'' | ||
