2psm: Difference between revisions
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[[Image:2psm.jpg|left|200px]] | [[Image:2psm.jpg|left|200px]] | ||
'''Crystal structure of Interleukin 15 in complex with Interleukin 15 receptor alpha''' | {{Structure | ||
|PDB= 2psm |SIZE=350|CAPTION= <scene name='initialview01'>2psm</scene>, resolution 2.19Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=BAM:BENZAMIDINE'>BAM</scene> | |||
|ACTIVITY= | |||
|GENE= Il15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), Il15ra ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | |||
}} | |||
'''Crystal structure of Interleukin 15 in complex with Interleukin 15 receptor alpha''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2PSM is a [ | 2PSM is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSM OCA]. | ||
==Reference== | ==Reference== | ||
Crystal Structure of the interleukin-15.interleukin-15 receptor alpha complex: insights into trans and cis presentation., Olsen SK, Ota N, Kishishita S, Kukimoto-Niino M, Murayama K, Uchiyama H, Toyama M, Terada T, Shirouzu M, Kanagawa O, Yokoyama S, J Biol Chem. 2007 Dec 21;282(51):37191-204. Epub 2007 Oct 18. PMID:[http:// | Crystal Structure of the interleukin-15.interleukin-15 receptor alpha complex: insights into trans and cis presentation., Olsen SK, Ota N, Kishishita S, Kukimoto-Niino M, Murayama K, Uchiyama H, Toyama M, Terada T, Shirouzu M, Kanagawa O, Yokoyama S, J Biol Chem. 2007 Dec 21;282(51):37191-204. Epub 2007 Oct 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17947230 17947230] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: golgi apparatus]] | [[Category: golgi apparatus]] | ||
[[Category: membrane]] | [[Category: membrane]] | ||
[[Category: national project on protein structural and functional | [[Category: national project on protein structural and functional analyse]] | ||
[[Category: nppsfa]] | [[Category: nppsfa]] | ||
[[Category: nucleus]] | [[Category: nucleus]] | ||
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[[Category: rsgi]] | [[Category: rsgi]] | ||
[[Category: secreted]] | [[Category: secreted]] | ||
[[Category: structural | [[Category: structural genomic]] | ||
[[Category: sushi]] | [[Category: sushi]] | ||
[[Category: transmembrane]] | [[Category: transmembrane]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:16:54 2008'' | ||
Revision as of 16:16, 20 March 2008
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| 2psm, resolution 2.19Å | |||||||||||||
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| Ligands: | BAM | ||||||||||||
| Gene: | Il15 (Mus musculus), Il15ra (Mus musculus) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of Interleukin 15 in complex with Interleukin 15 receptor alpha
Overview
Interleukin (IL)-15 is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity. IL-15 is unique among cytokines due to its participation in a trans signaling mechanism in which IL-15 receptor alpha (IL-15Ralpha) from one subset of cells presents IL-15 to neighboring IL-2Rbeta/gammac-expressing cells. Here we present the crystal structure of IL-15 in complex with the sushi domain of IL-15Ralpha. The structure reveals that the alpha receptor-binding epitope of IL-15 adopts a unique conformation, which, together with amino acid substitutions, permits specific interactions with IL-15Ralpha that account for the exceptionally high affinity of the IL-15.IL-15Ralpha complex. Interestingly, analysis of the topology of IL-15 and IL-15Ralpha at the IL-15.IL-15Ralpha interface suggests that IL-15 should be capable of participating in a cis signaling mechanism similar to that of the related cytokine IL-2. Indeed, we present biochemical data demonstrating that IL-15 is capable of efficiently signaling in cis through IL-15Ralpha and IL-2Rbeta/gammac expressed on the surface of a single cell. Based on our data we propose that cis presentation of IL-15 may be important in certain biological contexts and that flexibility of IL-15Ralpha permits IL-15 and its three receptor components to be assembled identically at the ligand-receptor interface whether IL-15 is presented in cis or trans. Finally, we have gained insights into IL-15.IL-15Ralpha.IL-2Rbeta.gammac quaternary complex assembly through the use of molecular modeling.
About this Structure
2PSM is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Crystal Structure of the interleukin-15.interleukin-15 receptor alpha complex: insights into trans and cis presentation., Olsen SK, Ota N, Kishishita S, Kukimoto-Niino M, Murayama K, Uchiyama H, Toyama M, Terada T, Shirouzu M, Kanagawa O, Yokoyama S, J Biol Chem. 2007 Dec 21;282(51):37191-204. Epub 2007 Oct 18. PMID:17947230
Page seeded by OCA on Thu Mar 20 18:16:54 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Mus musculus
- Protein complex
- Kanagawa, O.
- Kishishita, S.
- Kukimoto-Niino, M.
- Murayama, K.
- Olsen, S K.
- Ota, N.
- RSGI, RIKEN Structural Genomics/Proteomics Initiative.
- Shirouzu, M.
- Terada, T.
- Yokoyama, S.
- BAM
- Alternative splicing
- Cytokine
- Endoplasmic reticulum
- Glycoprotein
- Golgi apparatus
- Membrane
- National project on protein structural and functional analyse
- Nppsfa
- Nucleus
- Phosphorylation
- Receptor
- Riken structural genomics/proteomics initiative
- Rsgi
- Secreted
- Structural genomic
- Sushi
- Transmembrane
