4bwi: Difference between revisions
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==Structure of the phytochrome Cph2 from Synechocystis sp. PCC6803== | |||
<StructureSection load='4bwi' size='340' side='right' caption='[[4bwi]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4bwi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cyanobacterium_synechocystis Cyanobacterium synechocystis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BWI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BWI FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bwi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bwi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bwi RCSB], [http://www.ebi.ac.uk/pdbsum/4bwi PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Phytochromes are highly versatile photoreceptors, which occur ubiquitously in plants as well as in many light-responsive microorganisms. Here, photosynthetic cyanobacteria utilize up to three different phytochrome architectures, where only the plant-like and the single-domain cyanobacteriochromes are structurally characterized so far. Cph2 represents a third group in Synechocystis species and affects their capability of phototaxis by controlling c-di-GMP synthesis and degradation. The 2.6 A crystal structure of its red/far-red responsive photosensory module in the Pr state reveals a tandem-GAF bidomain that lacks the figure-of-eight knot of the plant/cph1 subfamily. Its covalently attached phycocyanobilin chromophore adopts a highly tilted ZZZssa conformation with a novel set of interactions between its propionates and the GAF1 domain. The tongue-like protrusion from the GAF2 domain interacts with the GAF1-bound chromophore via its conserved PRxSF, WxE, and WG/AG motifs. Mutagenesis showed that the tongue's integrity is indispensable for Pr-->Pfr photoconversion and involves a swap of the motifs' tryptophans within the tongue-GAF1 interface. This Trp switch is supposed to be a crucial element for the photochromicity of all multi-domain phytochromes. | |||
Structure of the cyanobacterial phytochrome 2 photosensor implies a tryptophan switch for phytochrome signaling.,Anders K, Daminelli-Widany G, Mroginski MA, von Stetten D, Essen LO J Biol Chem. 2013 Oct 30. PMID:24174528<ref>PMID:24174528</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
== | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Cyanobacterium synechocystis]] | [[Category: Cyanobacterium synechocystis]] | ||
[[Category: Anders, K | [[Category: Anders, K]] | ||
[[Category: Angerer, V | [[Category: Angerer, V]] | ||
[[Category: Essen, L O | [[Category: Essen, L O]] | ||
[[Category: Mroginski, M A | [[Category: Mroginski, M A]] | ||
[[Category: Stetten, D v | [[Category: Stetten, D v]] | ||
[[Category: Widany, G D | [[Category: Widany, G D]] | ||
[[Category: Pcb]] | [[Category: Pcb]] | ||
[[Category: Phycocyanobilin]] | [[Category: Phycocyanobilin]] | ||
[[Category: Red light photoreceptor]] | [[Category: Red light photoreceptor]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
