4ceh: Difference between revisions

Line 1:

~~'''Unreleased~~ structure~~'''~~

===Crystal structure of AddAB with a forked DNA substrate===

The ~~entry 4ceh~~ is ~~ON HOLD~~

==Function==

[[http://www.uniprot.org/uniprot/ADDA_BACSU ADDA_BACSU]] An essential component of the DNA double-stranded break repair machinery, the heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the B.subtilis chi site (5'-AGCGG-3') which transforms the enzyme from a helicase which degrades both DNA strands to one with only 5' -> 3' exonuclease activity. This generates a double-stranded DNA with a protruding 3'-terminated single-stranded tail suitable for the initiation of homologous recombination (chi fragment). The AddA nuclease domain in particular is required for chi fragment generation; this subunit has 3' -> 5' nuclease and helicase activity. RecA thread formation during DNA double-strand break repair requires RecJ or AddAB.<ref>PMID:8387145</ref> <ref>PMID:10756102</ref> <ref>PMID:17570399</ref> [[http://www.uniprot.org/uniprot/ADDB_BACSU ADDB_BACSU]] The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent single-stranded exonuclease, acting in both directions. Recognizes the B.subtilis chi site (5'-AGCGG-3') which transforms the enzyme from a helicase which degrades both DNA strands to one with only 5' to 3' exonuclease activity. This generates a double-stranded DNA with a protruding 3'-terminated single-stranded tail suitable for the initiation of homologous recombination (chi fragment). The AddB nuclease domain is not required for chi fragment generation; this subunit has 5' -> 3' nuclease activity. RecA thread formation during DNA double-strand break repair requires RecJ or AddAB.<ref>PMID:8387145</ref> <ref>PMID:10756102</ref> <ref>PMID:17570399</ref>

~~Authors~~: ~~Krajewski, W.W., Wilkinson, M., Fu, X~~.~~, Cronin, N~~.B.~~, Wigley, D~~.

==About this Structure==

[[4ceh]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CEH OCA].

~~Description~~: ~~Crystal structure of AddAB with a forked DNA substrate~~

==Reference==

[[Category: Cronin, N B.]]

[[Category: Fu, X.]]

[[Category: Krajewski, W W.]]

[[Category: Wigley, D.]]

[[Category: Wilkinson, M.]]

[[Category: Bacterial protein]]

[[Category: Binding site]]

[[Category: Dna break]]

[[Category: Dna helicase]]

[[Category: Dna repair]]

[[Category: Dna- binding protein]]

[[Category: Double-stranded]]

[[Category: Exodeoxyribonuclease v]]

[[Category: Exodeoxyribonuclease]]

[[Category: Helicase-nuclease]]

[[Category: Homologous recombination]]

[[Category: Hydrolase-dna complex]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+{{STRUCTURE_4ceh|  PDB=4ceh  |  SCENE=  }}
+===Crystal structure of AddAB with a forked DNA substrate===
-The entry 4ceh is ON HOLD
+==Function==
+[[http://www.uniprot.org/uniprot/ADDA_BACSU ADDA_BACSU]] An essential component of the DNA double-stranded break repair machinery, the heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the B.subtilis chi site (5'-AGCGG-3') which transforms the enzyme from a helicase which degrades both DNA strands to one with only 5' -> 3' exonuclease activity. This generates a double-stranded DNA with a protruding 3'-terminated single-stranded tail suitable for the initiation of homologous recombination (chi fragment). The AddA nuclease domain in particular is required for chi fragment generation; this subunit has 3' -> 5' nuclease and helicase activity. RecA thread formation during DNA double-strand break repair requires RecJ or AddAB.<ref>PMID:8387145</ref> <ref>PMID:10756102</ref> <ref>PMID:17570399</ref>  [[http://www.uniprot.org/uniprot/ADDB_BACSU ADDB_BACSU]] The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent single-stranded exonuclease, acting in both directions. Recognizes the B.subtilis chi site (5'-AGCGG-3') which transforms the enzyme from a helicase which degrades both DNA strands to one with only 5' to 3' exonuclease activity. This generates a double-stranded DNA with a protruding 3'-terminated single-stranded tail suitable for the initiation of homologous recombination (chi fragment). The AddB nuclease domain is not required for chi fragment generation; this subunit has 5' -> 3' nuclease activity. RecA thread formation during DNA double-strand break repair requires RecJ or AddAB.<ref>PMID:8387145</ref> <ref>PMID:10756102</ref> <ref>PMID:17570399</ref>
-Authors: Krajewski, W.W., Wilkinson, M., Fu, X., Cronin, N.B., Wigley, D.
+==About this Structure==
+[[4ceh]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CEH OCA].
-Description: Crystal structure of AddAB with a forked DNA substrate
+==Reference==
+<references group="xtra"/><references/>
+[[Category: Cronin, N B.]]
+[[Category: Fu, X.]]
+[[Category: Krajewski, W W.]]
+[[Category: Wigley, D.]]
+[[Category: Wilkinson, M.]]
+[[Category: Bacterial protein]]
+[[Category: Binding site]]
+[[Category: Dna break]]
+[[Category: Dna helicase]]
+[[Category: Dna repair]]
+[[Category: Dna- binding protein]]
+[[Category: Double-stranded]]
+[[Category: Exodeoxyribonuclease v]]
+[[Category: Exodeoxyribonuclease]]
+[[Category: Helicase-nuclease]]
+[[Category: Homologous recombination]]
+[[Category: Hydrolase-dna complex]]