Sandbox Reserved 777: Difference between revisions
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== Structure == | == Structure == | ||
The cytokine structure is an intercalated dimer of two identical polypeptide chains, each 178 amino acids in length.'''(4)''' The monomers are made up of six amphipathic helices, with two helices of one monomer interacting with four of the other monomer, creating two distinct domains. A four-helix bundle is made up of helices A, C, D and F’ (and A’, C’ D’ and F). This feature is a signature element of all helical cytokines.'''(5)''' The structure is stabilized by two disulfide bridges formed between the first and third cysteine residues and the second and fourth cysteine residues (Cys12 with Cys108, and Cys62 with Cys114). Reduction of the disulfide bonds in recombinant IL-10 has been shown to reduce helical content measured by circular dichroism and results in a lack of in vitro biological activity.'''(6)''' | The cytokine structure is an intercalated dimer of two identical polypeptide chains, each 178 amino acids in length.'''(4)''' The monomers are made up of six amphipathic helices, with two helices of one monomer interacting with four of the other monomer, creating two distinct domains.<Structure load='2H24' size='300' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' /> A four-helix bundle is made up of helices A, C, D and F’ (and A’, C’ D’ and F). This feature is a signature element of all helical cytokines.'''(5)''' The structure is stabilized by two disulfide bridges formed between the first and third cysteine residues and the second and fourth cysteine residues (Cys12 with Cys108, and Cys62 with Cys114). Reduction of the disulfide bonds in recombinant IL-10 has been shown to reduce helical content measured by circular dichroism and results in a lack of in vitro biological activity.'''(6)''' | ||
== Protein Expression == | == Protein Expression == | ||
