2qiy: Difference between revisions
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[[Image:2qiy.gif|left|200px]] | [[Image:2qiy.gif|left|200px]] | ||
'''yeast Deubiquitinase Ubp3 and Bre5 cofactor complex''' | {{Structure | ||
|PDB= 2qiy |SIZE=350|CAPTION= <scene name='initialview01'>2qiy</scene>, resolution 1.69Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Ubiquitin_thiolesterase Ubiquitin thiolesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.15 3.1.2.15] | |||
|GENE= BRE5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), UBP3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |||
}} | |||
'''yeast Deubiquitinase Ubp3 and Bre5 cofactor complex''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2QIY is a [ | 2QIY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QIY OCA]. | ||
==Reference== | ==Reference== | ||
Molecular basis for bre5 cofactor recognition by the ubp3 deubiquitylating enzyme., Li K, Ossareh-Nazari B, Liu X, Dargemont C, Marmorstein R, J Mol Biol. 2007 Sep 7;372(1):194-204. Epub 2007 Jun 27. PMID:[http:// | Molecular basis for bre5 cofactor recognition by the ubp3 deubiquitylating enzyme., Li K, Ossareh-Nazari B, Liu X, Dargemont C, Marmorstein R, J Mol Biol. 2007 Sep 7;372(1):194-204. Epub 2007 Jun 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17632125 17632125] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: phosphorylation]] | [[Category: phosphorylation]] | ||
[[Category: protein-protein recognition]] | [[Category: protein-protein recognition]] | ||
[[Category: rna-binding ]] | [[Category: rna-binding]] | ||
[[Category: signaling protein/hydrolase complex]] | [[Category: signaling protein/hydrolase complex]] | ||
[[Category: thiol protease]] | [[Category: thiol protease]] | ||
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[[Category: ubl conjugation pathway]] | [[Category: ubl conjugation pathway]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:26:17 2008'' | ||
Revision as of 16:26, 20 March 2008
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| 2qiy, resolution 1.69Å | |||||||||||||
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| Gene: | BRE5 (Saccharomyces cerevisiae), UBP3 (Saccharomyces cerevisiae) | ||||||||||||
| Activity: | Ubiquitin thiolesterase, with EC number 3.1.2.15 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
yeast Deubiquitinase Ubp3 and Bre5 cofactor complex
Overview
Yeast Ubp3 and its co-factor Bre5 form a deubiquitylation complex to regulate protein transport between the endoplasmic reticulum and Golgi compartments of the cell. A novel N-terminal domain of the Ubp3 catalytic subunit forms a complex with the NTF2-like domain of the Bre5 regulatory subunit. Here, we report the X-ray crystal structure of an Ubp3-Bre5 complex and show that it forms a symmetric hetero-tetrameric complex in which the Bre5 NTF2-like domain dimer interacts with two L-shaped beta-strand-turn-alpha-helix motifs of Ubp3. The Ubp3 N-terminal domain binds within a hydrophobic cavity on the surface of the Bre5 NTF2-like domain subunit with conserved residues within both proteins interacting predominantly through antiparallel beta-sheet hydrogen bonds and van der Waals contacts. Structure-based mutagenesis and functional studies confirm the significance of the observed interactions for Ubp3-Bre5 association in vitro and Ubp3 function in vivo. Comparison of the structure to other protein complexes with NTF2-like domains shows that the Ubp3-Bre5 interface is novel. Together, these studies provide new insights into Ubp3 recognition by Bre5 and into protein recognition by NTF2-like domains.
About this Structure
2QIY is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Molecular basis for bre5 cofactor recognition by the ubp3 deubiquitylating enzyme., Li K, Ossareh-Nazari B, Liu X, Dargemont C, Marmorstein R, J Mol Biol. 2007 Sep 7;372(1):194-204. Epub 2007 Jun 27. PMID:17632125
Page seeded by OCA on Thu Mar 20 18:26:17 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Protein complex
- Saccharomyces cerevisiae
- Ubiquitin thiolesterase
- Li, K.
- Liu, X.
- Marmorstein, R.
- Deubiquitylation
- Hydrolase
- Ntf2
- Phosphorylation
- Protein-protein recognition
- Rna-binding
- Signaling protein/hydrolase complex
- Thiol protease
- Ubiquitin-specific processing proteases(ubps)
- Ubl conjugation pathway
