1zia: Difference between revisions
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==Overview== | ==Overview== | ||
New crystals of a blue copper protein, pseudoazurin from denitrifier, Achromobacter cycloclastes IAM1013, have been obtained by means of vapor, diffusion with ammonium sulfate as a precipitant at pH 6.0 and 4 degrees, C. The crystals belong to the orthorhombic system, space group, P2(1)2(1)2(1), with unit cell dimensions of a = 56.69(2), b = 61.53(2), and c = 30.20(1) A. The asymmetric unit includes one molecule of, pseudoazurin with a Vm value of 2.04 A3/Da. The crystals are so stable, against X-ray irradiation that a complete data set up to 1.54 A has been, collected using a single native crystal. Solution of the structure was, performed by means of the Patterson search techniques, and the current, crystallographic R-factor is 17.5% at 3.0 A resolution. Refinement at, higher resolution . | New crystals of a blue copper protein, pseudoazurin from denitrifier, Achromobacter cycloclastes IAM1013, have been obtained by means of vapor, diffusion with ammonium sulfate as a precipitant at pH 6.0 and 4 degrees, C. The crystals belong to the orthorhombic system, space group, P2(1)2(1)2(1), with unit cell dimensions of a = 56.69(2), b = 61.53(2), and c = 30.20(1) A. The asymmetric unit includes one molecule of, pseudoazurin with a Vm value of 2.04 A3/Da. The crystals are so stable, against X-ray irradiation that a complete data set up to 1.54 A has been, collected using a single native crystal. Solution of the structure was, performed by means of the Patterson search techniques, and the current, crystallographic R-factor is 17.5% at 3.0 A resolution. Refinement at, higher resolution is in progress. | ||
==About this Structure== | ==About this Structure== | ||
1ZIA is a | 1ZIA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Achromobacter_cycloclastes Achromobacter cycloclastes] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: COP. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZIA OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: electron transport]] | [[Category: electron transport]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:36:16 2007'' | ||
