2qvw: Difference between revisions
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'''Structure of Giardia Dicer refined against twinned data''' | {{Structure | ||
|PDB= 2qvw |SIZE=350|CAPTION= <scene name='initialview01'>2qvw</scene>, resolution 3.000Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] | |||
|GENE= | |||
}} | |||
'''Structure of Giardia Dicer refined against twinned data''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2QVW is a [ | 2QVW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVW OCA]. | ||
==Reference== | ==Reference== | ||
An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer., MacRae IJ, Doudna JA, Acta Crystallogr D Biol Crystallogr. 2007 Sep;63(Pt 9):993-9. Epub 2007, Aug 17. PMID:[http:// | An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer., MacRae IJ, Doudna JA, Acta Crystallogr D Biol Crystallogr. 2007 Sep;63(Pt 9):993-9. Epub 2007, Aug 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17704568 17704568] | ||
[[Category: Giardia intestinalis]] | [[Category: Giardia intestinalis]] | ||
[[Category: Ribonuclease III]] | [[Category: Ribonuclease III]] | ||
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[[Category: rnai]] | [[Category: rnai]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:30:08 2008'' | ||
Revision as of 16:30, 20 March 2008
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| 2qvw, resolution 3.000Å | |||||||||||||
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| Ligands: | MN | ||||||||||||
| Activity: | Ribonuclease III, with EC number 3.1.26.3 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Structure of Giardia Dicer refined against twinned data
Overview
The crystal structure of the enzyme Dicer from Giardia intestinalis was solved to 3.3 A resolution by MAD using crystals belonging to space group P2(1)2(1)2 [Macrae et al. (2006), Science, 311, 195-198]. These crystals were derived from crystals that diffracted X-rays to 3.0 A resolution but were refractory to structure determination because they were twinned. It is shown here that the original Dicer crystals represent an unusual case of perfect pseudo-merohedral twinning of orthorhombic crystals. Before the twinning problem was overcome, it was possible to calculate a low-resolution electron-density map in space group P4(1) that was used to build a partial molecular model. Experimental phases were sufficient to identify heavy-atom sites that indicated space-group inconsistency, leading to identification of the true space group. This information guided the search for different crystallization conditions that yielded untwinned crystals and ultimately a fully interpretable electron-density map.
About this Structure
2QVW is a Single protein structure of sequence from Giardia intestinalis. Full crystallographic information is available from OCA.
Reference
An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer., MacRae IJ, Doudna JA, Acta Crystallogr D Biol Crystallogr. 2007 Sep;63(Pt 9):993-9. Epub 2007, Aug 17. PMID:17704568
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