4k0j: Difference between revisions
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{{Large structure}} | {{Large structure}} | ||
==X-ray crystal structure of a heavy metal efflux pump, crystal form I== | |||
<StructureSection load='4k0j' size='340' side='right' caption='[[4k0j]], [[Resolution|resolution]] 3.00Å' scene=''> | |||
{{ | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4k0j]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Cupriavidus_metallidurans_ch34 Cupriavidus metallidurans ch34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K0J FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4k0e|4k0e]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">zneA, Rmet_5329 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=266264 Cupriavidus metallidurans CH34])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k0j OCA], [http://pdbe.org/4k0j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4k0j RCSB], [http://www.ebi.ac.uk/pdbsum/4k0j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4k0j ProSAT]</span></td></tr> | |||
</table> | |||
{{Large structure}} | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Efflux pumps belonging to the ubiquitous resistance-nodulation-cell division (RND) superfamily transport substrates out of cells by coupling proton conduction across the membrane to a conformationally driven pumping cycle. The heavy metal-resistant bacteria Cupriavidus metallidurans CH34 relies notably on as many as 12 heavy metal efflux pumps of the RND superfamily. Here we show that C. metallidurans CH34 ZneA is a proton driven efflux pump specific for Zn(II), and that transport of substrates through the transmembrane domain may be electrogenic. We report two X-ray crystal structures of ZneA in intermediate transport conformations, at 3.0 and 3.7 A resolution. The trimeric ZneA structures capture protomer conformations that differ in the spatial arrangement and Zn(II) occupancies at a proximal and a distal substrate binding site. Structural comparison shows that transport of substrates through a tunnel that links the two binding sites, toward an exit portal, is mediated by the conformation of a short 14-aa loop. Taken together, the ZneA structures presented here provide mechanistic insights into the conformational changes required for substrate efflux by RND superfamily transporters. | |||
Structures of intermediate transport states of ZneA, a Zn(II)/proton antiporter.,Pak JE, Ekende EN, Kifle EG, O'Connell JD 3rd, De Angelis F, Tessema MB, Derfoufi KM, Robles-Colmenares Y, Robbins RA, Goormaghtigh E, Vandenbussche G, Stroud RM Proc Natl Acad Sci U S A. 2013 Nov 12;110(46):18484-9. doi:, 10.1073/pnas.1318705110. Epub 2013 Oct 30. PMID:24173033<ref>PMID:24173033</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
< | </div> | ||
<div class="pdbe-citations 4k0j" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Cupriavidus metallidurans ch34]] | [[Category: Cupriavidus metallidurans ch34]] | ||
[[Category: CSMP, Center for Structures of Membrane Proteins | [[Category: CSMP, Center for Structures of Membrane Proteins]] | ||
[[Category: Ekende, E Ngonlong | [[Category: Ekende, E Ngonlong]] | ||
[[Category: Pak, J E | [[Category: Pak, J E]] | ||
[[Category: Stroud, R M | [[Category: Stroud, R M]] | ||
[[Category: Vandenbussche, G | [[Category: Vandenbussche, G]] | ||
[[Category: Center for structures of membrane protein]] | [[Category: Center for structures of membrane protein]] | ||
[[Category: Csmp]] | [[Category: Csmp]] | ||
Revision as of 00:08, 5 August 2016
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X-ray crystal structure of a heavy metal efflux pump, crystal form I
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