Sandbox Reserved 777: Difference between revisions
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== Structure == | == Structure == | ||
[[Image:4helixbundle.png|300px|left|thumb|The four-helix bundle]] | [[Image:4helixbundle.png|300px|left|thumb|The four-helix bundle. Image built in Pymol using PDB 2H24]] | ||
The cytokine structure is an intercalated dimer of two identical polypeptide chains, each 178 amino acids in length.'''(4)''' The monomers are made up of six amphipathic helices, with two helices of one monomer interacting with four of the other monomer, creating two distinct domains.A four-helix bundle is made up of helices A, C, D and F’ (and A’, C’ D’ and F). This feature is a signature element of all helical cytokines.'''(5)''' The structure is stabilized by <scene name='56/564053/Il-10_disulfide_bonds/2'>two disulfide bridges</scene> formed between the first and third cysteine residues and the second and fourth cysteine residues (Cys12 with Cys108, and Cys62 with Cys114). In the image, the disulfide bridges are shown in yellow. Reduction of the disulfide bonds in recombinant IL-10 has been shown to reduce helical content measured by circular dichroism and results in a lack of in vitro biological activity.'''(6)'''[[Image:1ilk bio r 500.jpg |right|thumb| another view of the biologically active homodimer]] | The cytokine structure is an intercalated dimer of two identical polypeptide chains, each 178 amino acids in length.'''(4)''' The monomers are made up of six amphipathic helices, with two helices of one monomer interacting with four of the other monomer, creating two distinct domains.A four-helix bundle is made up of helices A, C, D and F’ (and A’, C’ D’ and F). This feature is a signature element of all helical cytokines.'''(5)''' The structure is stabilized by <scene name='56/564053/Il-10_disulfide_bonds/2'>two disulfide bridges</scene> formed between the first and third cysteine residues and the second and fourth cysteine residues (Cys12 with Cys108, and Cys62 with Cys114). In the image, the disulfide bridges are shown in yellow. Reduction of the disulfide bonds in recombinant IL-10 has been shown to reduce helical content measured by circular dichroism and results in a lack of in vitro biological activity.'''(6)'''[[Image:1ilk bio r 500.jpg |right|thumb| another view of the biologically active homodimer]] | ||
