Sandbox Reserved 760: Difference between revisions

The first method for crystallization of nitrogenase was put forward by Burns R.C., Holsten R.D. and Hardy R.W., then was modified by Shah V.K. and Brill W.J. and was simplified by the 7th Laboratory, Institute of Botany, The Chinese Academy of Sciences. But the crystals gotten from these laboratories were small and needle-shaped therefore wasn’t suitable for X-ray diffraction analysis. Weininger M.S. and Mortenson L. E. attempted to do X-ray diffraction analysis of MoFe protein crystals grown by using microdialysis method but they did not succeed in analyzing the crystallographic structure of MoFe protein. Later Kim and Rees succeeded in growing the big crystals of Azotobacter vinelandii nitrogenase by the microcapillary batch method and in analyzing of these crystals by X-ray diffraction. Since then, the liquid/liquid diffusion method has been usually used for crystal growth of nitrogenase proteins.

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