Sandbox Reserved 770: Difference between revisions
From Proteopedia
Jump to navigationJump to search
Bryan Toton (talk | contribs) |
Bryan Toton (talk | contribs) |
||
| Line 41: | Line 41: | ||
==Structure== | ==Structure== | ||
[[Image:Seahorse.png|thumb|left|Figure 2. Subunit B (colored red) of PAL tetramer in both ribbon and surface representations. Cinnamic acid, a product formed from by L-Phe catalysis, is also shown in this figure. <ref name=rutgers>http://maptest.rutgers.edu/drupal/?q=node/408</ref>.]] | [[Image:Seahorse.png|thumb|left|Figure 2. Subunit B (colored red) of PAL tetramer in both ribbon and surface representations. Cinnamic acid, a product formed from by L-Phe catalysis, is also shown in this figure. <ref name=rutgers>http://maptest.rutgers.edu/drupal/?q=node/408</ref>.]] | ||
Phenylalanine ammonia lyase enzyme is a dimer composed of two identical subunits. <ref name="crystallization">http://ci.nii.ac.jp/els/110006324658.pdf?id=ART0008332067&type=pdf&lang=en&host=cinii&order_no=&ppv_type=0&lang_sw=&no=1386328093&cp=</ref> Each subunit of PAL from ''R. toruloides'' assume a "seahorse" shape by interlocking head-to-tail, creating overlapping regions with two adjacent subunits, as shown by Figure 2. These overlapping regions maximize interactions between subunits, giving rise to the formation of the tightly assembled tetramer. Formation of the tetramer buries 58% of their combined surfaces. <ref name=crystal>http://pubs.acs.org.prox.lib.ncsu.edu/doi/pdfplus/10.1021/bi049053%2B</ref> | Phenylalanine ammonia lyase enzyme is a dimer composed of two identical subunits. <ref name="crystallization">http://ci.nii.ac.jp/els/110006324658.pdf?id=ART0008332067&type=pdf&lang=en&host=cinii&order_no=&ppv_type=0&lang_sw=&no=1386328093&cp=</ref> Each subunit of PAL from ''R. toruloides'' assume a "seahorse" shape by interlocking head-to-tail, creating overlapping regions with two adjacent subunits, as shown by Figure 2. These overlapping regions maximize interactions between subunits, giving rise to the formation of the tightly assembled tetramer. Formation of the tetramer buries 58% of their combined surfaces. <ref name=crystal>http://pubs.acs.org.prox.lib.ncsu.edu/doi/pdfplus/10.1021/bi049053%2B</ref> Of the 66 interactions between adjacent subunits, 25 hydrogen bonding interactions exists between Asp & Glu carboxylate oxygens and NH2 & OH moieties, including a prominent band of Asp & Glu interactions with Arg side chains between subunits nearby the central bundle of helices. | ||
==References== | ==References== | ||
<references /> | <references /> | ||
