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[[Image:MIO.png|thumb|left|250px|Figure 5. Cofactor MIO Structure]]

The central core within each monomer of PAL contains three central alpha helices of triple-coiled coils (Helix 3,Helix 4, and Helix 7), improving its rigidity similar to fibrous proteins in keratin. The three core helices are oriented with similarly aligned dipoles to create an electro-positive platform for cofactor 3,5-dihydro-5-methylidene-4H-imidazol-4-one (MIO, Figure 5.)to anchor through noncovalent bonding, shown in Figure 4b. Covalent linkages between cofactor MIO and PAL backbone act to direct the C-terminus of MIO to an alpha helix's N-terminus, creating a loop, thus the helix's positive pole points toward the cofactor and the active site.

The central core within each monomer of PAL contains three central alpha helices of triple-coiled coils (Helix 3,Helix 4, and Helix 7), improving its rigidity similar to fibrous proteins in keratin. The three core helices are oriented with similarly aligned dipoles to create an electro-positive platform for cofactor 3,5-dihydro-5-methylidene-4H-imidazol-4-one (MIO, Figure 5.) to anchor through noncovalent bonding, shown in Figure 4b. Covalent linkages between cofactor MIO and PAL backbone act to direct the C-terminus of MIO to an alpha helix's N-terminus, creating a loop, thus the helix's positive pole points toward the cofactor and the active site.

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@@ Line 59: / Line 59: @@
 [[Image:MIO.png|thumb|left|250px|Figure 5. Cofactor MIO Structure]]
-The central core within each monomer of PAL contains three central alpha helices of triple-coiled coils (Helix 3,Helix 4, and Helix 7), improving its rigidity similar to fibrous proteins in keratin. The three core helices are oriented with similarly aligned dipoles to create an electro-positive platform for cofactor 3,5-dihydro-5-methylidene-4H-imidazol-4-one (MIO, Figure 5.)to anchor through noncovalent bonding, shown in Figure 4b. Covalent linkages between cofactor MIO and PAL backbone act to direct the C-terminus of MIO to an alpha helix's N-terminus, creating a loop, thus the helix's positive pole points toward the cofactor and the active site.
+The central core within each monomer of PAL contains three central alpha helices of triple-coiled coils (Helix 3,Helix 4, and Helix 7), improving its rigidity similar to fibrous proteins in keratin. The three core helices are oriented with similarly aligned dipoles to create an electro-positive platform for cofactor 3,5-dihydro-5-methylidene-4H-imidazol-4-one (MIO, Figure 5.) to anchor through noncovalent bonding, shown in Figure 4b. Covalent linkages between cofactor MIO and PAL backbone act to direct the C-terminus of MIO to an alpha helix's N-terminus, creating a loop, thus the helix's positive pole points toward the cofactor and the active site.
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