Sandbox Reserved 770: Difference between revisions
From Proteopedia
Jump to navigationJump to search
Bryan Toton (talk | contribs) |
Bryan Toton (talk | contribs) |
||
| Line 87: | Line 87: | ||
[[Image:L-Phe_PAL_t-cinnamic_acid.png|thumbnail|right|600px|Figure 13. L-Phenylalanine substrate with k1 being the slow first step creating the stable carbanion, followed by a rapid product synsthesis in products CIN and Ammonia in Phenylalanine Ammonia Lyase enzyme.]] | [[Image:L-Phe_PAL_t-cinnamic_acid.png|thumbnail|right|600px|Figure 13. L-Phenylalanine substrate with k1 being the slow first step creating the stable carbanion, followed by a rapid product synsthesis in products CIN and Ammonia in Phenylalanine Ammonia Lyase enzyme.]] | ||
'''Rate Law of E1cB Mechanism''': Second Order Kinetics observed | <nowiki>'''Rate Law of E1cB Mechanism''': Second Order Kinetics observed</nowiki> | ||
1) E1cB anion : anion is stable; rapid first step, followed by the slow formation of products (k1>>k2). | <nowiki>1) E1cB anion : anion is stable; rapid first step, followed by the slow formation of products (k1>>k2).</nowiki> | ||
2) E1cB rev : first step is reversible, formation of product slower than reforming the starting material, this again results from a slow second step (k-1>>k2). | <nowiki>2) E1cB rev : first step is reversible, formation of product slower than reforming the starting material, this again results from a slow second step (k-1>>k2).</nowiki> | ||
3) E1cB irr : first step is slow (formation of carbanion intermediate); but once formed, the product quickly follows (k2>>k1,k-1). This leads to an irreversible first step. | <nowiki>3) E1cB irr : first step is slow (formation of carbanion intermediate); but once formed, the product quickly follows (k2>>k1,k-1). This leads to an irreversible first step.</nowiki> | ||
==Methods Used to Solve Structure== | ==Methods Used to Solve Structure== | ||
