3w8w: Difference between revisions
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==The crystal structure of EncM== | |||
===The crystal structure of EncM | <StructureSection load='3w8w' size='340' side='right' caption='[[3w8w]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3w8w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/'streptomyces_maritimus' 'streptomyces maritimus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W8W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W8W FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3w8x|3w8x]], [[3w8y|3w8y]], [[3w8z|3w8z]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">encM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=115828 'Streptomyces maritimus'])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w8w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3w8w RCSB], [http://www.ebi.ac.uk/pdbsum/3w8w PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Flavoproteins catalyse a diversity of fundamental redox reactions and are one of the most studied enzyme families. As monooxygenases, they are universally thought to control oxygenation by means of a peroxyflavin species that transfers a single atom of molecular oxygen to an organic substrate. Here we report that the bacterial flavoenzyme EncM catalyses the peroxyflavin-independent oxygenation-dehydrogenation dual oxidation of a highly reactive poly(beta-carbonyl). The crystal structure of EncM with bound substrate mimics and isotope labelling studies reveal previously unknown flavin redox biochemistry. We show that EncM maintains an unexpected stable flavin-oxygenating species, proposed to be a flavin-N5-oxide, to promote substrate oxidation and trigger a rare Favorskii-type rearrangement that is central to the biosynthesis of the antibiotic enterocin. This work provides new insight into the fine-tuning of the flavin cofactor in offsetting the innate reactivity of a polyketide substrate to direct its efficient electrocyclization. | |||
Flavin-mediated dual oxidation controls an enzymatic Favorskii-type rearrangement.,Teufel R, Miyanaga A, Michaudel Q, Stull F, Louie G, Noel JP, Baran PS, Palfey B, Moore BS Nature. 2013 Nov 28;503(7477):552-6. doi: 10.1038/nature12643. Epub 2013 Oct 27. PMID:24162851<ref>PMID:24162851</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Streptomyces maritimus]] | [[Category: Streptomyces maritimus]] | ||
[[Category: Baran, P S | [[Category: Baran, P S]] | ||
[[Category: Louie, G | [[Category: Louie, G]] | ||
[[Category: Michaudel, Q | [[Category: Michaudel, Q]] | ||
[[Category: Miyanaga, A | [[Category: Miyanaga, A]] | ||
[[Category: Moore, B S | [[Category: Moore, B S]] | ||
[[Category: Noel, J P | [[Category: Noel, J P]] | ||
[[Category: Palfey, B | [[Category: Palfey, B]] | ||
[[Category: Stull, F | [[Category: Stull, F]] | ||
[[Category: Teufel, R | [[Category: Teufel, R]] | ||
[[Category: Flavin binding]] | [[Category: Flavin binding]] | ||
[[Category: Monooxygenase]] | [[Category: Monooxygenase]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
