4cht: Difference between revisions
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{{STRUCTURE_4cht| PDB=4cht | SCENE= }} | |||
===Crystal structure of the human topoisomerase III alpha-RMI1 complex with bound calcium ion=== | |||
The | ==Function== | ||
[[http://www.uniprot.org/uniprot/TOP3A_HUMAN TOP3A_HUMAN]] Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.<ref>PMID:8622991</ref> <ref>PMID:20445207</ref> [[http://www.uniprot.org/uniprot/RMI1_HUMAN RMI1_HUMAN]] Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability.<ref>PMID:15775963</ref> <ref>PMID:16595695</ref> <ref>PMID:16537486</ref> | |||
==About this Structure== | |||
[[4cht]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CHT OCA]. | |||
==Reference== | |||
<references group="xtra"/><references/> | |||
[[Category: DNA topoisomerase]] | |||
[[Category: Bocquet, N.]] | |||
[[Category: Bunker, R D.]] | |||
[[Category: Thoma, N H.]] | |||
[[Category: Cell cycle]] | |||
[[Category: Decatenation]] | |||
[[Category: Double holliday junction dissolution]] | |||
[[Category: Minimal dissolvasome]] | |||
