4btq: Difference between revisions
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==Coordinates of the bacteriophage phi6 capsid subunits fitted into the cryoEM map EMD-1206== | |||
<StructureSection load='4btq' size='340' side='right' caption='[[4btq]], [[Resolution|resolution]] 7.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4btq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpph6 Bpph6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BTQ FirstGlance]. <br> | |||
==Function== | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4btg|4btg]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4btq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4btq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4btq RCSB], [http://www.ebi.ac.uk/pdbsum/4btq PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/P1_BPPH6 P1_BPPH6]] P1 is the major inner capsid (core) protein of the polyhedral procapsid, which is responsible for genomic replication and transcription. Forms a dodecahedral shell from 60 asymmetric dimers. Binds to RNA and may be involved in genomic packaging. | [[http://www.uniprot.org/uniprot/P1_BPPH6 P1_BPPH6]] P1 is the major inner capsid (core) protein of the polyhedral procapsid, which is responsible for genomic replication and transcription. Forms a dodecahedral shell from 60 asymmetric dimers. Binds to RNA and may be involved in genomic packaging. | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The cystovirus varphi6 shares several distinct features with other double-stranded RNA (dsRNA) viruses, including the human pathogen, rotavirus: segmented genomes, nonequivalent packing of 120 subunits in its icosahedral capsid, and capsids as compartments for transcription and replication. varphi6 assembles as a dodecahedral procapsid that undergoes major conformational changes as it matures into the spherical capsid. We determined the crystal structure of the capsid protein, P1, revealing a flattened trapezoid subunit with an alpha-helical fold. We also solved the procapsid with cryo-electron microscopy to comparable resolution. Fitting the crystal structure into the procapsid disclosed substantial conformational differences between the two P1 conformers. Maturation via two intermediate states involves remodeling on a similar scale, besides huge rigid-body rotations. The capsid structure and its stepwise maturation that is coupled to sequential packaging of three RNA segments sets the cystoviruses apart from other dsRNA viruses as a dynamic molecular machine. | |||
Subunit Folds and Maturation Pathway of a dsRNA Virus Capsid.,Nemecek D, Boura E, Wu W, Cheng N, Plevka P, Qiao J, Mindich L, Heymann JB, Hurley JH, Steven AC Structure. 2013 Aug 6;21(8):1374-83. doi: 10.1016/j.str.2013.06.007. Epub 2013, Jul 25. PMID:23891288<ref>PMID:23891288</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
[[Category: | == References == | ||
[[Category: Boura, E | <references/> | ||
[[Category: Cheng, N | __TOC__ | ||
[[Category: Heymann, J B | </StructureSection> | ||
[[Category: Hurley, J H | [[Category: Bpph6]] | ||
[[Category: Mindich, L | [[Category: Boura, E]] | ||
[[Category: Nemecek, D | [[Category: Cheng, N]] | ||
[[Category: Plevka, P | [[Category: Heymann, J B]] | ||
[[Category: Qiao, J | [[Category: Hurley, J H]] | ||
[[Category: Steven, A C | [[Category: Mindich, L]] | ||
[[Category: Wu, W | [[Category: Nemecek, D]] | ||
[[Category: Plevka, P]] | |||
[[Category: Qiao, J]] | |||
[[Category: Steven, A C]] | |||
[[Category: Wu, W]] | |||
[[Category: Cystoviridae]] | [[Category: Cystoviridae]] | ||
[[Category: Flexible fitting]] | [[Category: Flexible fitting]] | ||
[[Category: Procapsid structure]] | [[Category: Procapsid structure]] | ||
[[Category: Viral protein]] | [[Category: Viral protein]] | ||
