2uuv: Difference between revisions

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[[Image:2uuv.gif|left|200px]]<br /><applet load="2uuv" size="350" color="white" frame="true" align="right" spinBox="true"
[[Image:2uuv.gif|left|200px]]
caption="2uuv, resolution 1.99&Aring;" />
 
'''ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE IN P1'''<br />
{{Structure
|PDB= 2uuv |SIZE=350|CAPTION= <scene name='initialview01'>2uuv</scene>, resolution 1.99&Aring;
|SITE= <scene name='pdbsite=AC1:Pl3+Binding+Site+For+Chain+D'>AC1</scene>
|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> and <scene name='pdbligand=PL3:HEXADECAN-1-OL'>PL3</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/Alkylglycerone-phosphate_synthase Alkylglycerone-phosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.26 2.5.1.26]
|GENE=
}}
 
'''ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE IN P1'''
 


==Overview==
==Overview==
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==About this Structure==
==About this Structure==
2UUV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum] with <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=PL3:'>PL3</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alkylglycerone-phosphate_synthase Alkylglycerone-phosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.26 2.5.1.26] Known structural/functional Site: <scene name='pdbsite=AC1:Pl3+Binding+Site+For+Chain+D'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUV OCA].  
2UUV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUV OCA].  


==Reference==
==Reference==
The crucial step in ether phospholipid biosynthesis: structural basis of a noncanonical reaction associated with a peroxisomal disorder., Razeto A, Mattiroli F, Carpanelli E, Aliverti A, Pandini V, Coda A, Mattevi A, Structure. 2007 Jun;15(6):683-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17562315 17562315]
The crucial step in ether phospholipid biosynthesis: structural basis of a noncanonical reaction associated with a peroxisomal disorder., Razeto A, Mattiroli F, Carpanelli E, Aliverti A, Pandini V, Coda A, Mattevi A, Structure. 2007 Jun;15(6):683-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17562315 17562315]
[[Category: Alkylglycerone-phosphate synthase]]
[[Category: Alkylglycerone-phosphate synthase]]
[[Category: Dictyostelium discoideum]]
[[Category: Dictyostelium discoideum]]
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[[Category: FAD]]
[[Category: FAD]]
[[Category: PL3]]
[[Category: PL3]]
[[Category: biosynthesis of phospholipids]]
[[Category: biosynthesis of phospholipid]]
[[Category: fad]]
[[Category: fad]]
[[Category: flavin]]
[[Category: flavin]]
Line 30: Line 39:
[[Category: peroxisomal disorder]]
[[Category: peroxisomal disorder]]
[[Category: peroxisome]]
[[Category: peroxisome]]
[[Category: plasmalogens]]
[[Category: plasmalogen]]
[[Category: rhizomelic chondrodysplasia punctata]]
[[Category: rhizomelic chondrodysplasia punctata]]
[[Category: transferase]]
[[Category: transferase]]


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:50:26 2008''
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:40:03 2008''

Revision as of 16:40, 20 March 2008

File:2uuv.gif


Drag the structure with the mouse to rotate
2uuv, resolution 1.99Å
Sites: AC1
Ligands: FAD and PL3
Activity: Alkylglycerone-phosphate synthase, with EC number 2.5.1.26
Coordinates: save as pdb, mmCIF, xml



ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE IN P1


Overview

Ether phospholipids are essential constituents of eukaryotic cell membranes. Rhizomelic chondrodysplasia punctata type 3 is a severe peroxisomal disorder caused by inborn deficiency of alkyldihydroxyacetonephosphate synthase (ADPS). The enzyme carries out the most characteristic step in ether phospholipid biosynthesis: formation of the ether bond. The crystal structure of ADPS from Dictyostelium discoideum shows a fatty-alcohol molecule bound in a narrow hydrophobic tunnel, specific for aliphatic chains of 16 carbons. Access to the tunnel is controlled by a flexible loop and a gating helix at the protein-membrane interface. Structural and mutagenesis investigations identify a cluster of hydrophilic catalytic residues, including an essential tyrosine, possibly involved in substrate proton abstraction, and the arginine that is mutated in ADPS-deficient patients. We propose that ether bond formation might be orchestrated through a covalent imine intermediate with the flavin, accounting for the noncanonical employment of a flavin cofactor in a nonredox reaction.

About this Structure

2UUV is a Single protein structure of sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA.

Reference

The crucial step in ether phospholipid biosynthesis: structural basis of a noncanonical reaction associated with a peroxisomal disorder., Razeto A, Mattiroli F, Carpanelli E, Aliverti A, Pandini V, Coda A, Mattevi A, Structure. 2007 Jun;15(6):683-92. PMID:17562315

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