2bh2: Difference between revisions
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==Overview== | ==Overview== | ||
A single base (U1939) within E. coli 23S ribosomal RNA is methylated by, its dedicated enzyme, RumA. The structure of, RumA/RNA/S-adenosylhomocysteine uncovers the mechanism for achieving, unique selectivity. The single-stranded substrate is "refolded" on the, enzyme into a compact conformation with six key intra-RNA interactions., The RNA substrate contributes directly to catalysis. In addition to the, target base, a second base is "flipped out" from the core loop to stack, against the adenine of the cofactor S-adenosylhomocysteine. Nucleotides in, permuted sequence order are stacked into the site vacated by the everted, target U1939 and compensate for the energetic penalty of base eversion., The 3' hairpin segment of the RNA binds distal to the active site and, provides binding energy .. | A single base (U1939) within E. coli 23S ribosomal RNA is methylated by, its dedicated enzyme, RumA. The structure of, RumA/RNA/S-adenosylhomocysteine uncovers the mechanism for achieving, unique selectivity. The single-stranded substrate is "refolded" on the, enzyme into a compact conformation with six key intra-RNA interactions., The RNA substrate contributes directly to catalysis. In addition to the, target base, a second base is "flipped out" from the core loop to stack, against the adenine of the cofactor S-adenosylhomocysteine. Nucleotides in, permuted sequence order are stacked into the site vacated by the everted, target U1939 and compensate for the energetic penalty of base eversion., The 3' hairpin segment of the RNA binds distal to the active site and, provides binding energy that contributes to enhanced catalytic efficiency., Active collaboration of RNA in catalysis leads us to conclude that RumA, and its substrate RNA may reflect features from the earliest RNA-protein, era. | ||
==About this Structure== | ==About this Structure== | ||
2BH2 is a | 2BH2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SAH and SF4 as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BH2 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:54:56 2007'' | ||
