4bw9: Difference between revisions
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==PylRS Y306G, Y384F, I405R mutant in complex with AMP-PNP== | |||
<StructureSection load='4bw9' size='340' side='right' caption='[[4bw9]], [[Resolution|resolution]] 2.35Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4bw9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_2053 Dsm 2053]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BW9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BW9 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bwa|4bwa]]</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrrolysine--tRNA(Pyl)_ligase Pyrrolysine--tRNA(Pyl) ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.26 6.1.1.26] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bw9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bw9 RCSB], [http://www.ebi.ac.uk/pdbsum/4bw9 PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/PYLS_METMA PYLS_METMA]] Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Three for two: By using a Methanosarcina mazei PylRS triple mutant (Y306G, Y384F, I405R) the incorporation of two new exo-norbornene-containing pyrrolysine analogues was achieved. X-ray crystallographic analysis led to the identification of the crucial structural elements involved in substrate recognition by the evolved synthetase. | |||
Structural Insights into Incorporation of Norbornene Amino Acids for Click Modification of Proteins.,Schneider S, Gattner MJ, Vrabel M, Flugel V, Lopez-Carrillo V, Prill S, Carell T Chembiochem. 2013 Sep 11. doi: 10.1002/cbic.201300435. PMID:24027216<ref>PMID:24027216</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Pyrrolysyl-tRNA synthetase|Pyrrolysyl-tRNA synthetase]] | *[[Pyrrolysyl-tRNA synthetase|Pyrrolysyl-tRNA synthetase]] | ||
== References == | |||
== | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Dsm 2053]] | [[Category: Dsm 2053]] | ||
[[Category: Carell, T | [[Category: Carell, T]] | ||
[[Category: Fluegel, V | [[Category: Fluegel, V]] | ||
[[Category: Gattner, M J | [[Category: Gattner, M J]] | ||
[[Category: Lopez-Carillo, V | [[Category: Lopez-Carillo, V]] | ||
[[Category: Schneider, S | [[Category: Schneider, S]] | ||
[[Category: Vrabel, M | [[Category: Vrabel, M]] | ||
[[Category: Ligase]] | [[Category: Ligase]] | ||
