Sandbox Reserved 828: Difference between revisions
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Gyrase is '''coded by two differents contiguous genes gyrA and gyrB''' as it is a 350 kDa '''A<sub>2</sub>B<sub>2</sub> heterotetramers''' of two A proteins and two B proteins. | Gyrase is '''coded by two differents contiguous genes gyrA and gyrB''' as it is a 350 kDa '''A<sub>2</sub>B<sub>2</sub> heterotetramers''' of two A proteins and two B proteins. | ||
The A protein breaks and religates DNA . | |||
The B protein has ATPase activity | The A protein breaks and religates DNA. | ||
The B protein has ATPase activity. | |||
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[[Image:Gyrasegene.jpg]] | [[Image:Gyrasegene.jpg]] | ||
'''The A protein''' breaks and religates DNA as the DNA cleavage core and the CTD lies on it. | '''The A protein''' breaks and religates DNA as the DNA cleavage core and the CTD lies on it. The smallest fragment capable of catalyzing DNA breakage and reunion is between residues 7 and 523 of A sequence, it is the gyrA59 (59kDa). | ||
The gyrA | The gyrA N-ter domain is called '''breakage and reunion domain'''. It is composed of two domains at the <scene name='56/568026/Head/2'>head</scene> region : | ||
*a winged-helix-turn-helix domain or <scene name='56/568026/Whd/1'>winged helix domain (WHD</scene>) where lies the catalytic tyrosines: the active-site tyrosines <scene name='56/568026/Tyr122/1'>(Tyr 122)</scene> are on loops at either end of the dimer interface. | *a winged-helix-turn-helix domain or <scene name='56/568026/Whd/1'>winged helix domain (WHD</scene>) where lies the catalytic tyrosines: the active-site tyrosines <scene name='56/568026/Tyr122/1'>(Tyr 122)</scene> are on loops at either end of the dimer interface. | ||
