2v95: Difference between revisions
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[[Image:2v95.gif|left|200px]] | [[Image:2v95.gif|left|200px]] | ||
'''STRUTURE OF CORTICOSTEROID-BINDING GLOBULIN IN COMPLEX WITH CORTISOL''' | {{Structure | ||
|PDB= 2v95 |SIZE=350|CAPTION= <scene name='initialview01'>2v95</scene>, resolution 1.93Å | |||
|SITE= <scene name='pdbsite=AC1:Pdn+Binding+Site+For+Chain+A'>AC1</scene> | |||
|LIGAND= <scene name='pdbligand=PDN:17,21-DIHYDROXYPREGNA-1,4-DIENE-3,11,20-TRIONE'>PDN</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''STRUTURE OF CORTICOSTEROID-BINDING GLOBULIN IN COMPLEX WITH CORTISOL''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2V95 is a [ | 2V95 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. This structure supersedes the now removed PDB entry 2V6D. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V95 OCA]. | ||
==Reference== | ==Reference== | ||
Corticosteroid-binding globulin, a structural basis for steroid transport and proteinase-triggered release., Klieber MA, Underhill C, Hammond GL, Muller YA, J Biol Chem. 2007 Oct 5;282(40):29594-603. Epub 2007 Jul 19. PMID:[http:// | Corticosteroid-binding globulin, a structural basis for steroid transport and proteinase-triggered release., Klieber MA, Underhill C, Hammond GL, Muller YA, J Biol Chem. 2007 Oct 5;282(40):29594-603. Epub 2007 Jul 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17644521 17644521] | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: cbg]] | [[Category: cbg]] | ||
[[Category: corticosteroid-binding globulin transport protein]] | [[Category: corticosteroid-binding globulin transport protein]] | ||
[[Category: | [[Category: glucocorticoid]] | ||
[[Category: glycoprotein]] | [[Category: glycoprotein]] | ||
[[Category: lipid-binding]] | [[Category: lipid-binding]] | ||
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[[Category: transport]] | [[Category: transport]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:44:24 2008'' | ||
Revision as of 16:44, 20 March 2008
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| 2v95, resolution 1.93Å | |||||||||||||
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| Sites: | AC1 | ||||||||||||
| Ligands: | PDN | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
STRUTURE OF CORTICOSTEROID-BINDING GLOBULIN IN COMPLEX WITH CORTISOL
Overview
Corticosteroid-binding globulin (CBG) is a serine proteinase inhibitor (serpin) family member that transports glucocorticoids in blood and regulates their access to target cells. The 1.9A crystal structure of rat CBG shows that its steroid-binding site resembles the thyroxin-binding site in the related serpin, thyroxin-binding globulin, and mutagenesis studies have confirmed the contributions of key residues that constitute the steroid-binding pocket. Unlike thyroxin-bound thyroxin-binding globulin, the cortisol-bound CBG displays an "active" serpin conformation with the proteinase-sensitive, reactive center loop (RCL) fully expelled from the regulatory beta-sheet A. Moreover, the CBG structure allows us to predict that complete insertion of the proteolytically cleaved RCL into the serpin fold occurs in concert with a displacement and unwinding of helix D that would disrupt the steroid-binding site. This allosteric coupling between RCL positioning and occupancy of the CBG steroid-binding site, which resembles the ligand (glycosamino-glycan)-dependent activation of the thrombin inhibitory serpins heparin cofactor II and anti-thrombin RCLs, ensures both optimal recognition of CBG by target proteinases and efficient release of steroid to sites of action.
About this Structure
2V95 is a Single protein structure of sequence from Rattus norvegicus. This structure supersedes the now removed PDB entry 2V6D. Full crystallographic information is available from OCA.
Reference
Corticosteroid-binding globulin, a structural basis for steroid transport and proteinase-triggered release., Klieber MA, Underhill C, Hammond GL, Muller YA, J Biol Chem. 2007 Oct 5;282(40):29594-603. Epub 2007 Jul 19. PMID:17644521
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