2veb: Difference between revisions
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'''HIGH RESOLUTION STRUCTURE OF PROTOGLOBIN FROM METHANOSARCINA ACETIVORANS C2A''' | {{Structure | ||
|PDB= 2veb |SIZE=350|CAPTION= <scene name='initialview01'>2veb</scene>, resolution 1.30Å | |||
|SITE= <scene name='pdbsite=AC1:Hem+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Po4+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Po4+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Gol+Binding+Site+For+Chain+A'>AC4</scene> and <scene name='pdbsite=AC5:Oxy+Binding+Site+For+Chain+A'>AC5</scene> | |||
|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''HIGH RESOLUTION STRUCTURE OF PROTOGLOBIN FROM METHANOSARCINA ACETIVORANS C2A''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2VEB is a [ | 2VEB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanosarcina_acetivorans Methanosarcina acetivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VEB OCA]. | ||
==Reference== | ==Reference== | ||
Archaeal protoglobin structure indicates new ligand diffusion paths and modulation of haem-reactivity., Nardini M, Pesce A, Thijs L, Saito JA, Dewilde S, Alam M, Ascenzi P, Coletta M, Ciaccio C, Moens L, Bolognesi M, EMBO Rep. 2008 Feb;9(2):157-63. Epub 2008 Jan 11. PMID:[http:// | Archaeal protoglobin structure indicates new ligand diffusion paths and modulation of haem-reactivity., Nardini M, Pesce A, Thijs L, Saito JA, Dewilde S, Alam M, Ascenzi P, Coletta M, Ciaccio C, Moens L, Bolognesi M, EMBO Rep. 2008 Feb;9(2):157-63. Epub 2008 Jan 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18188182 18188182] | ||
[[Category: Methanosarcina acetivorans]] | [[Category: Methanosarcina acetivorans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: hemoprotein structure]] | [[Category: hemoprotein structure]] | ||
[[Category: methanogenesis]] | [[Category: methanogenesis]] | ||
[[Category: protein matrix | [[Category: protein matrix tunnel]] | ||
[[Category: protoglobin]] | [[Category: protoglobin]] | ||
[[Category: transport protein]] | [[Category: transport protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:45:41 2008'' | ||
Revision as of 16:45, 20 March 2008
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| 2veb, resolution 1.30Å | |||||||||||||
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| Sites: | AC1, AC2, AC3, AC4 and AC5 | ||||||||||||
| Ligands: | PO4, HEM, OXY and GOL | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
HIGH RESOLUTION STRUCTURE OF PROTOGLOBIN FROM METHANOSARCINA ACETIVORANS C2A
Overview
The structural adaptability of the globin fold has been highlighted by the recent discovery of the 2-on-2 haemoglobins, of neuroglobin and cytoglobin. Protoglobin from Methanosarcina acetivorans C2A-a strictly anaerobic methanogenic Archaea-is, to the best of our knowledge, the latest entry adding new variability and functional complexity to the haemoglobin (Hb) superfamily. Here, we report the 1.3 A crystal structure of oxygenated M. acetivorans protoglobin, together with the first insight into its ligand-binding properties. We show that, contrary to all known globins, protoglobin-specific loops and an amino-terminal extension completely bury the haem within the protein matrix. Access of O(2), CO and NO to the haem is granted by the protoglobin-specific apolar tunnels reaching the haem distal site from locations at the B/G and B/E helix interfaces. Functionally, M. acetivorans dimeric protoglobin shows a selectivity ratio for O(2)/CO binding to the haem that favours O(2) ligation and anticooperativity in ligand binding. Both properties are exceptional within the Hb superfamily.
About this Structure
2VEB is a Single protein structure of sequence from Methanosarcina acetivorans. Full crystallographic information is available from OCA.
Reference
Archaeal protoglobin structure indicates new ligand diffusion paths and modulation of haem-reactivity., Nardini M, Pesce A, Thijs L, Saito JA, Dewilde S, Alam M, Ascenzi P, Coletta M, Ciaccio C, Moens L, Bolognesi M, EMBO Rep. 2008 Feb;9(2):157-63. Epub 2008 Jan 11. PMID:18188182
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