Sandbox Reserved 822: Difference between revisions
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The PH domain of PDK1 reveals a structural variation of a standard PH domain fold with an additional 'bud' at the N-terminus. | The PH domain of PDK1 reveals a structural variation of a standard PH domain fold with an additional 'bud' at the N-terminus. | ||
[[Image:Topology_Diagram_of_PH_domain_of_PDK1.JPG|left|320px|thumb|Fig.1 Topology diagram of the PH domain of PDK1. The colors of the different sections correspond to the coloring of the Jmol structure applet. <ref> PMID: 524332 </ref>]] | [[Image:Topology_Diagram_of_PH_domain_of_PDK1.JPG|left|320px|thumb|Fig.1 Topology diagram of the PH domain of PDK1. The colors of the different sections correspond to the coloring of the Jmol structure applet. <ref name="Structural"> PMID: 524332 </ref>]] | ||
The standard PH domain fold consists of mainly three different sections: | The standard PH domain fold consists of mainly three different sections: | ||
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*On the other side of the barrel three variable loops (VL 1-3) form a bowl-like structure which is lined by positively charged residues, consituting the phosphoinositide-binding site. | *On the other side of the barrel three variable loops (VL 1-3) form a bowl-like structure which is lined by positively charged residues, consituting the phosphoinositide-binding site. | ||
The PH domain of PDK1 possesses an additional extension (<scene name='56/568020/Bud/1'>'bud'</scene>) N-terminal to the standard PH domain fold. This bud forms two additional β strands and one α helix and is an integral part of the overall fold. The two β strands β1' and β2'extend the β1 - β4 sheet in an antiparallel fashion through the formation of β sheet hydrogen bonds between β2'and β1. The α helix packs against this newly formed six stranded β sheet forming an additional <scene name='56/568020/Hydrophobic_core/1'>hydrophobic core</scene> outside of the standard PH domain fold. The bud binds to the β1 - β4 sheet by several additional hydrophobic contacts and buries more than 30% of the surface of the standard PH domain fold. <ref | The PH domain of PDK1 possesses an additional extension (<scene name='56/568020/Bud/1'>'bud'</scene>) N-terminal to the standard PH domain fold. This bud forms two additional β strands and one α helix and is an integral part of the overall fold. The two β strands β1' and β2'extend the β1 - β4 sheet in an antiparallel fashion through the formation of β sheet hydrogen bonds between β2'and β1. The α helix packs against this newly formed six stranded β sheet forming an additional <scene name='56/568020/Hydrophobic_core/1'>hydrophobic core</scene> outside of the standard PH domain fold. The bud binds to the β1 - β4 sheet by several additional hydrophobic contacts and buries more than 30% of the surface of the standard PH domain fold. <ref name="Structural" /> | ||
