2bjf: Difference between revisions
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==Overview== | ==Overview== | ||
Bacterial bile salt hydrolases catalyze the degradation of conjugated bile, acids in the mammalian gut. The crystal structures of conjugated bile acid, hydrolase (CBAH) from Clostridium perfringens as apoenzyme and in complex, with taurodeoxycholate that was hydrolyzed to the reaction products, taurine and deoxycholate are described here at 2.1 and 1.7 A resolution, respectively. The crystal structures reveal close relationship between, CBAH and penicillin V acylase from Bacillus sphaericus. This similarity, together with the N-terminal cysteine classifies CBAH as a member of the, N-terminal nucleophile (Ntn) hydrolase superfamily. Both crystal, structures show an identical homotetrameric organization with dihedral, (D(2) or 222) point group symmetry. The structure analysis of C., . | Bacterial bile salt hydrolases catalyze the degradation of conjugated bile, acids in the mammalian gut. The crystal structures of conjugated bile acid, hydrolase (CBAH) from Clostridium perfringens as apoenzyme and in complex, with taurodeoxycholate that was hydrolyzed to the reaction products, taurine and deoxycholate are described here at 2.1 and 1.7 A resolution, respectively. The crystal structures reveal close relationship between, CBAH and penicillin V acylase from Bacillus sphaericus. This similarity, together with the N-terminal cysteine classifies CBAH as a member of the, N-terminal nucleophile (Ntn) hydrolase superfamily. Both crystal, structures show an identical homotetrameric organization with dihedral, (D(2) or 222) point group symmetry. The structure analysis of C., perfringens CBAH identifies critical residues in catalysis, substrate, recognition, and tetramer formation which may serve in further biochemical, characterization of bile acid hydrolases. | ||
==About this Structure== | ==About this Structure== | ||
2BJF is a | 2BJF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens] with DXC, TAU and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Choloylglycine_hydrolase Choloylglycine hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.24 3.5.1.24] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BJF OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: ntn-hydrolase]] | [[Category: ntn-hydrolase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:32:10 2007'' | ||
