4jp7: Difference between revisions
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==High resolution structure of a papaya barwin-like protein (crystal form 2)== | |||
<StructureSection load='4jp7' size='340' side='right' caption='[[4jp7]], [[Resolution|resolution]] 1.05Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4jp7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Carica_papaya Carica papaya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JP7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JP7 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4jp6|4jp6]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jp7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jp7 RCSB], [http://www.ebi.ac.uk/pdbsum/4jp7 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The first crystal structure of a barwin-like protein, named carwin, has been determined at high resolution by single-wavelength anomalous diffraction (SAD) phasing using the six intrinsic S atoms present in the protein. The barwin-like protein was purified from Carica papaya latex and crystallized in the orthorhombic space group P212121. Using in-house Cu Kalpha X-ray radiation, 16 cumulative diffraction data sets were acquired to increase the signal-to-noise level and thereby the anomalous scattering signal. A sequence-database search on the papaya genome identified two carwin isoforms of 122 residues in length, both containing six S atoms that yield an estimated Bijvoet ratio of 0.93% at 1.54 A wavelength. A systematic analysis of data quality and redundancy was performed to assess the capacity to locate the S atoms and to phase the data. It was observed that the crystal decay was low during data collection and that successful S-SAD phasing could be obtained with a relatively low data multiplicity of about 7. Using a synchrotron source, high-resolution data (1 A) were collected from two different crystal forms of the papaya latex carwin. The refined structures showed a central beta-barrel of six strands surrounded by several alpha-helices and loops. The beta-barrel of carwin appears to be a common structural module that is shared within several other unrelated proteins. Finally, the possible biological function of the protein is discussed. | |||
High-resolution structure of a papaya plant-defence barwin-like protein solved by in-house sulfur-SAD phasing.,Huet J, Teinkela Mbosso EJ, Soror S, Meyer F, Looze Y, Wintjens R, Wohlkonig A Acta Crystallogr D Biol Crystallogr. 2013 Oct;69(Pt 10):2017-26. doi:, 10.1107/S0907444913018015. Epub 2013 Sep 20. PMID:24100320<ref>PMID:24100320</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Carica papaya]] | [[Category: Carica papaya]] | ||
[[Category: Wintjens, R | [[Category: Wintjens, R]] | ||
[[Category: Wohlkonig, A | [[Category: Wohlkonig, A]] | ||
[[Category: Barwin family]] | [[Category: Barwin family]] | ||
[[Category: Double-psi beta barrel]] | [[Category: Double-psi beta barrel]] | ||
[[Category: Pathogenesis-related protein of family 4]] | [[Category: Pathogenesis-related protein of family 4]] | ||
[[Category: Unknown function]] | [[Category: Unknown function]] | ||
Revision as of 18:29, 21 December 2014
High resolution structure of a papaya barwin-like protein (crystal form 2)
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